Development of a HPLC-MS/MS method for the simultaneous determination of nifedipine and lidocaine in human plasma.

Autor: Grigoriev A; Bioanalytical Laboratory CSU 'Analytical Spectrometry', Russia; Bioanalytical Laboratory CSU 'Analytical Spectrometry,' Peter the Great St. Petersburg Polytechnic University, Box 27, Gzhatskaya str. 27, 198220, St. Petersburg, Russia. Electronic address: a.grigoriev@csuas.ru., Nikitina A; Bioanalytical Laboratory CSU 'Analytical Spectrometry', Russia., Yaroshenko I; Bioanalytical Laboratory CSU 'Analytical Spectrometry', Russia., Sidorova A; Bioanalytical Laboratory CSU 'Analytical Spectrometry', Russia; Bioanalytical Laboratory CSU 'Analytical Spectrometry,' Peter the Great St. Petersburg Polytechnic University, Box 27, Gzhatskaya str. 27, 198220, St. Petersburg, Russia.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2016 Nov 30; Vol. 131, pp. 13-19. Date of Electronic Publication: 2016 Aug 05.
DOI: 10.1016/j.jpba.2016.08.004
Abstrakt: The method for simultaneous determination of nifedipine (NIF) and lidocaine (LID) in human plasma by one-step sample preparation has been developed for the first time. Due to the photosensitivity of nifedipine and its low plasma concentrations a precise and reliable method was required. The method involved liquid-liquid extraction (methyl tert-butyl ether, MTBE), and 10μL of the resulting sample was analyzed by HPLC-MS/MS. Chromatographic separation was achieved on an YMC-Triart C18 HPLC column (100×2.0mm; S-5μm 12nm). The mobile phase was methanol:water, 60:40 (v/v) and contained 0.15% acetic acid. The linearity of the method was established in the concentration ranges of 0.5-50ng/mL for NIF and 1.0-500ng/mL for LID. Photodestruction of NIF under ambient light was evaluated. The validated method was successfully applied to analyze human plasma samples after rectal application of the drug (1g) containing 2.0% LID and 0.3% NIF.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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