(E)-9-oxooctadec-10-en-12-ynoic acid from Ixora brachiata Roxb. increases glucose uptake in L6 myotubes by activating the PI3K pathway.

Autor: Thakkar CS; Screening - Metabolic Disorders Translational Unit, Piramal Enterprises Limited, 1A - Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, Maharashtra, India., Kshirsagar RR; Discovery Analytical Sciences Department, Piramal Enterprises Limited, 1A - Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, Maharashtra, India., Naik VR; Natural Products, Piramal Enterprises Limited, 1A - Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, Maharashtra, India., Ghosh AR; Centre for Infectious Diseases & Control, School of BioSciences and Technology, VIT University, Vellore 632014, India., Vijayakumar V; Centre for Organic and Medicinal Chemistry, VIT University, Vellore 632014, India., Saklani A; Natural Products, Piramal Enterprises Limited, 1A - Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, Maharashtra, India., Kulkarni-Almeida AA; Screening - Metabolic Disorders Translational Unit, Piramal Enterprises Limited, 1A - Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, Maharashtra, India. Electronic address: asha.almeida@gmail.com.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2016 Oct; Vol. 114, pp. 26-33. Date of Electronic Publication: 2016 Aug 10.
DOI: 10.1016/j.fitote.2016.08.004
Abstrakt: In an attempt to discover new scaffolds for anti-diabetic activity from plants, we screened extracts from Ixora brachiata Roxb. for their effect on glucose uptake in L6 myotubes. The petroleum (PE) extract of the plant showed a significant increase in insulin stimulated glucose uptake by L6 myotubes. The bioactivity guided fractionation of the crude extract yielded a compound (E)-9-oxooctadec-10-en-12-ynoic acid (OEA). The compound induced a dose dependent increase in insulin stimulated glucose uptake in L6 myotubes with an EC 50 of 22.96μM. OEA also increased the phosphorylation of IRS-1, Akt and AS160 leading to increased GLUT4 translocation to the plasma membrane indicating that it promotes insulin stimulated glucose uptake in L6 myotubes by activating the PI3K pathway.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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