Transplanted Senescent Cells Induce an Osteoarthritis-Like Condition in Mice.

Autor:	Xu M; Robert and Arlene Kogod Center on Aging., Bradley EW; Department of Orthopedic Surgery., Weivoda MM; Robert and Arlene Kogod Center on Aging., Hwang SM; Mayo Medical School, and., Pirtskhalava T; Robert and Arlene Kogod Center on Aging., Decklever T; Department of Radiology, Mayo Clinic, Rochester, Minnesota., Curran GL; Department of Radiology, Mayo Clinic, Rochester, Minnesota., Ogrodnik M; Robert and Arlene Kogod Center on Aging.; Newcastle University Institute for Ageing and Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK., Jurk D; Newcastle University Institute for Ageing and Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK., Johnson KO; Robert and Arlene Kogod Center on Aging., Lowe V; Department of Radiology, Mayo Clinic, Rochester, Minnesota., Tchkonia T; Robert and Arlene Kogod Center on Aging., Westendorf JJ; Department of Orthopedic Surgery.; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN., Kirkland JL; Robert and Arlene Kogod Center on Aging.
Jazyk:	angličtina
Zdroj:	The journals of gerontology. Series A, Biological sciences and medical sciences [J Gerontol A Biol Sci Med Sci] 2017 Jun 01; Vol. 72 (6), pp. 780-785.
DOI:	10.1093/gerona/glw154
Abstrakt:	Osteoarthritis (OA) is the leading form of arthritis in the elderly, causing pain, disability, and immobility. OA has been associated with accumulation of senescent cells in or near joints. However, evidence for a causal link between OA and cellular senescence is lacking. Here, we present a novel senescent cell transplantation model involving injection of small numbers of senescent or nonsenescent cells from the ear cartilage of luciferase-expressing mice into the knee joint area of wild-type mice. By using bioluminescence and 18FDG PET imaging, we could track the injected cells in vivo for more than 10 days. Transplanting senescent cells into the knee region caused leg pain, impaired mobility, and radiographic and histological changes suggestive of OA. Transplanting nonsenescent cells had less of these effects. Thus, senescent cells can induce an OA-like state and targeting senescent cells could be a promising strategy for treating OA. (© The Author 2016. Published by Oxford University Press on behalf of The Gerontological Society of America.)
Databáze:	MEDLINE
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