| Autor: |
Aprea J; DFG-Research Center and Cluster of Excellence for Regenerative Therapies; Dresden, Germany; Authors are equal contributing joint-first authors., Lesche M; Deep Sequencing Group, Biotechnology Center; Dresden, Germany; Authors are equal contributing joint-first authors., Massalini S; DFG-Research Center and Cluster of Excellence for Regenerative Therapies ; Dresden, Germany., Prenninger S; DFG-Research Center and Cluster of Excellence for Regenerative Therapies ; Dresden, Germany., Alexopoulou D; Deep Sequencing Group, Biotechnology Center ; Dresden, Germany., Dahl A; Deep Sequencing Group, Biotechnology Center ; Dresden, Germany., Hiller M; Max Planck Institute of Molecular Cell Biology and Genetics; Dresden, Germany; Max Planck Institute for the Physics of Complex Systems; Dresden, Germany., Calegari F; DFG-Research Center and Cluster of Excellence for Regenerative Therapies ; Dresden, Germany. |
| Abstrakt: |
Long non-coding (lnc)RNAs play key roles in many biological processes. Elucidating the function of lncRNAs in cell type specification during organ development requires knowledge about their expression in individual progenitor types rather than in whole tissues. To achieve this during cortical development, we used a dual-reporter mouse line to isolate coexisting proliferating neural stem cells, differentiating neurogenic progenitors and newborn neurons and assessed the expression of lncRNAs by paired-end, high-throughput sequencing. We identified 379 genomic loci encoding novel lncRNAs and performed a comprehensive assessment of cell-specific expression patterns for all, annotated and novel, lncRNAs described to date. Our study provides a powerful new resource for studying these elusive transcripts during stem cell commitment and neurogenesis. |
