New Methyloceanibacter diversity from North Sea sediments includes methanotroph containing solely the soluble methane monooxygenase.

Autor: Vekeman B; Department of Biochemistry and Microbiology, Laboratory of Microbiology (LM-UGent), Ghent University, Karel Lodewijck Ledeganckstraat 35, Gent, 9000, Belgium., Kerckhof FM; Center for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, Gent, 9000, Belgium., Cremers G; Department of Microbiology, IWWR, Radboud University Nijmegen, Heyendaalseweg 135, AJ Nijmegen, 6525, The Netherlands., de Vos P; Department of Biochemistry and Microbiology, Laboratory of Microbiology (LM-UGent), Ghent University, Karel Lodewijck Ledeganckstraat 35, Gent, 9000, Belgium., Vandamme P; Department of Biochemistry and Microbiology, Laboratory of Microbiology (LM-UGent), Ghent University, Karel Lodewijck Ledeganckstraat 35, Gent, 9000, Belgium.; BCCM/LMG Bacteria Collection, Ghent University, Karel Lodewijck Ledeganckstraat 35, Gent, 9000, Belgium., Boon N; Center for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, Gent, 9000, Belgium., Op den Camp HJ; Department of Microbiology, IWWR, Radboud University Nijmegen, Heyendaalseweg 135, AJ Nijmegen, 6525, The Netherlands., Heylen K; Department of Biochemistry and Microbiology, Laboratory of Microbiology (LM-UGent), Ghent University, Karel Lodewijck Ledeganckstraat 35, Gent, 9000, Belgium.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2016 Dec; Vol. 18 (12), pp. 4523-4536. Date of Electronic Publication: 2016 Sep 09.
DOI: 10.1111/1462-2920.13485
Abstrakt: Marine methylotrophs play a key role in the global carbon cycle by metabolizing reduced one-carbon compounds that are found in high concentrations in marine environments. Genome, physiology and diversity studies have been greatly facilitated by the numerous model organisms brought into culture. However, the availability of marine representatives remains poor. Here, we report the isolation of four novel species from North Sea sediment enrichments closely related to the Alphaproteobacterium Methyloceanibacter caenitepidi. Each of the newly isolated Methyloceanibacter species exhibited a clear genome sequence divergence which was reflected in physiological differences. Notably one strain R-67174 was capable of oxidizing methane as sole source of carbon and energy using solely a soluble methane monooxygenase and represents the first marine Alphaproteobacterial methanotroph brought into culture. Differences in maximum cell density of >1.5 orders of magnitude were observed. Furthermore, three strains were capable of producing nitrous oxide from nitrate. Together, these findings highlight the metabolic and physiologic variability within closely related Methyloceanibacter species and provide a new understanding of the physiological basis of marine methylotrophy.
(© 2016 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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