Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements.

Autor:	Nguyen NT; Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia., McInturf SA; Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia., Mendoza-Cózatl DG; Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia; mendozacozatld@missouri.edu.
Jazyk:	angličtina
Zdroj:	Journal of visualized experiments : JoVE [J Vis Exp] 2016 Jul 13 (113). Date of Electronic Publication: 2016 Jul 13.
DOI:	10.3791/54317
Abstrakt:	Hydroponic systems have been utilized as one of the standard methods for plant biology research and are also used in commercial production for several crops, including lettuce and tomato. Within the plant research community, numerous hydroponic systems have been designed to study plant responses to biotic and abiotic stresses. Here we present a hydroponic protocol that can be easily implemented in laboratories interested in pursuing studies on plant mineral nutrition. This protocol describes the hydroponic system set up in detail and the preparation of plant material for successful experiments. Most of the materials described in this protocol can be found outside scientific supply companies, making the set up for hydroponic experiments less expensive and convenient. The use of a hydroponic growth system is most advantageous in situations where the nutrient media need to be well controlled and when intact roots need to be harvested for downstream applications. We also demonstrate how nutrient concentrations can be modified to induce plant responses to both essential nutrients and toxic non-essential elements.
Databáze:	MEDLINE
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