Antimalarial potential of leaves of Chenopodium ambrosioides L.

Autor: Cysne DN; Laboratory of Immunophysiology, Department of Pathology, Center for Biological and Health Sciences, Federal University of Maranhão (UFMA), 65080-805, São Luís, Brazil., Fortes TS; Laboratory of Immunophysiology, Department of Pathology, Center for Biological and Health Sciences, Federal University of Maranhão (UFMA), 65080-805, São Luís, Brazil., Reis AS; Laboratory of Immunophysiology, Department of Pathology, Center for Biological and Health Sciences, Federal University of Maranhão (UFMA), 65080-805, São Luís, Brazil.; Department of Parasitology, Laboratory of Experimental Immunoparasitology, Institute of Biomedical Sciences, University of São Paulo (USP), 05508-000, São Paulo, SP, Brazil.; Medical College, Center of Social Sciences, Health and Technology, Federal University of Maranhão (UFMA), 65900-410, Imperatriz, Brazil., de Paulo Ribeiro B; Laboratory of Immunophysiology, Department of Pathology, Center for Biological and Health Sciences, Federal University of Maranhão (UFMA), 65080-805, São Luís, Brazil., Dos Santos Ferreira A; Laboratory of Biotechnology Applied to Health, Oswaldo Cruz Foundation (Fiocruz Rondônia), 76812-245, Porto Velho, RO, Brazil., do Amaral FM; Department of Pharmacy, Laboratory of Herbal Medicine, Center of Biological Sciences and Health, Federal University of Maranhão (UFMA), 65085-580, São Luís, Brazil., Guerra RN; Laboratory of Immunophysiology, Department of Pathology, Center for Biological and Health Sciences, Federal University of Maranhão (UFMA), 65080-805, São Luís, Brazil., Marinho CR; Department of Parasitology, Laboratory of Experimental Immunoparasitology, Institute of Biomedical Sciences, University of São Paulo (USP), 05508-000, São Paulo, SP, Brazil., Nicolete R; Laboratory of Biotechnology Applied to Health, Oswaldo Cruz Foundation (Fiocruz Rondônia), 76812-245, Porto Velho, RO, Brazil.; Oswaldo Cruz Foundation (Fiocruz-Ceará), 60175-047, Fortaleza, CE, Brazil., Nascimento FR; Laboratory of Immunophysiology, Department of Pathology, Center for Biological and Health Sciences, Federal University of Maranhão (UFMA), 65080-805, São Luís, Brazil. nascimentofrf@yahoo.com.br.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2016 Nov; Vol. 115 (11), pp. 4327-4334. Date of Electronic Publication: 2016 Aug 05.
DOI: 10.1007/s00436-016-5216-x
Abstrakt: In an effort to identify novel therapeutic alternatives for the treatment of malaria, the present study evaluated the antimalarial effect of the crude hydroalcoholic extract (HCE) from the leaves of Chenopodium ambrosioides L. For this purpose, the molecular affinity between the total proteins from erythrocytes infected with Plasmodium falciparum and HCE or chloroquine was evaluated by surface plasmon resonance (SPR). Subsequently, the plasmodicidal potential of HCE was assessed in a P. falciparum culture. Using BALB/c mice infected with Plasmodium berghei intraperitoneally (ip.), we evaluated the effects of ip. treatment, for three consecutive days (day 7, 8, and 9 after infection), with chloroquine (45 mg/kg) or HCE (5 mg/kg), considering the survival index and the parasitaemia. The groups were compared to an untreated control group that receives only PBS at the same periods. The results indicated that HCE could bind to the total proteins of infected erythrocytes and could inhibit the parasite growth in vitro (IC 50  = 25.4 g/mL). The in vivo therapeutic treatment with HCE increased the survival and decreased the parasitaemia in the infected animals. Therefore, the HCE treatment exhibited a significant antiplasmodial effect and may be considered as a potential candidate for the development of new antimalarial drugs.
Databáze: MEDLINE
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