Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid.

Autor: Ekiz A; Department of Pharmacology, School of Pharmacy, Marmara University, Istanbul, Turkey., Özdemir-Kumral ZN; Department of Physiology, School of Medicine, Marmara University, Istanbul, Turkey., Erşahin M; Department of Neurosurgery, School of Medicine, Medeniyet University, Istanbul, Turkey., Tuğtepe H; Department of Pediatric Surgery, School of Medicine, Marmara University, Istanbul, Turkey., Öğünç AV; Vocational School of Health Related Professions, Marmara University, Istanbul, Turkey., Akakın D; Department of Histology and Embryology, School of Medicine, Marmara University, Istanbul, Turkey., Kıran D; Department of Histology and Embryology, School of Medicine, Marmara University, Istanbul, Turkey., Özsavcı D; Department of Biochemistry, School of Pharmacy, Marmara University, Istanbul, Turkey., Biber N; Department of Neurosurgery, Haydarpasa Numune Teaching and Research Hospital, Istanbul, Turkey., Hakan T; Department of Neurosurgery, Okan University, Vocational School of Health Services, Kolan International Hospital, Istanbul, Turkey., Yeğen BÇ; Department of Physiology, School of Medicine, Marmara University, Istanbul, Turkey., Şener G; Department of Pharmacology, School of Pharmacy, Marmara University, Istanbul, Turkey., Toklu HZ; Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, Florida.; Geriatric Research Education and Clinical Center, Malcom Randall Veterans Affairs Medical Center, Gainesville, Florida.
Jazyk: angličtina
Zdroj: Neurourology and urodynamics [Neurourol Urodyn] 2017 Apr; Vol. 36 (4), pp. 1061-1068. Date of Electronic Publication: 2016 Aug 04.
DOI: 10.1002/nau.23083
Abstrakt: Background & Aim: Alpha lipoic acid (LA) was shown to exert neuroprotection in trauma-induced spinal cord injury (SCI), which is frequently associated with urinary bladder complaints in patients with SCI. Accordingly, the protective effects of LA on biochemical and histological changes in bladder as well as functional studies were assessed.
Methods: Wistar albino rats were divided as control, SCI, and LA (50 mg/kg/day, ip) treated SCI groups (SCI+LA). The standard weight-drop (100 g/cm force at T10) method was used to induce a moderately severe SCI. One week after the injury, neurological examination was performed and the rats were decapitated. Bladder samples were taken for histological examination, functional (isolated tissue bath) studies, and for the measurement of biochemical parameters (malondialdehyde, MDA; gluthathione, GSH; nerve growth factor, NGF; caspase-3, luminol and lucigenin chemiluminescences).
Results: SCI caused a significant (P < 0.001) increase in the detrusor muscle thickness. It increased the contractility responses to carbachol and relaxation responses to papaverine (P < 0.05-0.001). There were also significant alterations in MDA, caspase-3, luminol, and lucigenin chemiluminescences with concomitant decreases in NGF and GSH (P < 0.05). LA treatment reversed histological and functional (contraction and relaxation responses) changes induced by SCI (P < 0.05-0.001), but no significant recovery was observed in the impaired neurological functions.
Conclusion: These results indicate that LA have a beneficial effect in improving the bladder tonus via its antioxidant and anti-inflammatory actions following SCI.
(© 2016 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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