Deployment of a Reverse Transcription Loop-Mediated Isothermal Amplification Test for Ebola Virus Surveillance in Remote Areas in Guinea.

Autor:	Kurosaki Y; Institute of Tropical Medicine., Magassouba N; Laboratoire des Fièvres Hémorragiques en Guinée Hôpital National Donka, Service des Maladies Infectieuses et Tropicales Université Gamal Abdel Nasser de Conakry., Bah HA; Laboratoire des Fièvres Hémorragiques en Guinée Hôpital National Donka, Service des Maladies Infectieuses et Tropicales., Soropogui B; Laboratoire des Fièvres Hémorragiques en Guinée Hôpital National Donka, Service des Maladies Infectieuses et Tropicales., Doré A; Laboratoire des Fièvres Hémorragiques en Guinée Hôpital National Donka, Service des Maladies Infectieuses et Tropicales., Kourouma F; Laboratoire des Fièvres Hémorragiques en Guinée Hôpital National Donka, Service des Maladies Infectieuses et Tropicales., Cherif MS; Institute of Tropical Medicine., Keita S; Ministry of Health, Conakry, Guinea., Yasuda J; Institute of Tropical Medicine Graduate School of Biomedical Sciences, Nagasaki University, Japan.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2016 Oct 15; Vol. 214 (suppl 3), pp. S229-S233. Date of Electronic Publication: 2016 Aug 01.
DOI:	10.1093/infdis/jiw255
Abstrakt:	To strengthen the laboratory diagnostic capacity for Ebola virus disease (EVD) in the remote areas of Guinea, we deployed a mobile field laboratory and implemented reverse transcription loop-mediated isothermal amplification (RT-LAMP) for postmortem testing. We tested 896 oral swab specimens and 21 serum samples, using both RT-LAMP and reverse transcription-polymerase chain reaction (RT-PCR). Neither test yielded a positive result, and the results from RT-LAMP and RT-PCR were consistent. More than 95% of the samples were tested within 2 days of sample collection. These results highlight the usefulness of the RT-LAMP assay as an EVD diagnostic testing method in the field or remote areas. (© The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.)
Databáze:	MEDLINE
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