Individual Differences in Behavioural Despair Predict Brain GSK-3beta Expression in Mice: The Power of a Modified Swim Test.

Autor:	Strekalova T; Department of Neuroscience, School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, Netherlands., Markova N; Department of Neuroscience, School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, Netherlands; Laboratory of Biomolecular Screening, Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severnii Proezd 1, Chernogolovka, Moscow 142432, Russia; Laboratory of Cognitive Dysfunctions, Institute of General Pathology and Pathophysiology, Baltiiskaya Street 8, Moscow 125315, Russia., Shevtsova E; Laboratory of Biomolecular Screening, Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severnii Proezd 1, Chernogolovka, Moscow 142432, Russia., Zubareva O; Department of Physiology, Federal State Budgetary Scientific Institution 'Institute of Experimental Medicine', Akademika Pavlova Street 12, Saint-Petersburg 197022, Russia., Bakhmet A; Department of Human Anatomy, I.M. Sechenov First Moscow State Medical University, Mokhovaya Street 11-10, Moscow 125009, Russia., Steinbusch HM; Department of Neuroscience, School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, Netherlands., Bachurin S; Laboratory of Biomolecular Screening, Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severnii Proezd 1, Chernogolovka, Moscow 142432, Russia., Lesch KP; Department of Neuroscience, School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, Netherlands; Division of Molecular Psychiatry, Laboratory of Translational Neuroscience, Department of Psychiatry, Psychosomatics and Psychotherapy, University of Wuerzburg, Fuechsleinstrasse 15, 97080 Wuerzburg, Germany.
Jazyk:	angličtina
Zdroj:	Neural plasticity [Neural Plast] 2016; Vol. 2016, pp. 5098591. Date of Electronic Publication: 2016 Jul 05.
DOI:	10.1155/2016/5098591
Abstrakt:	While deficient brain plasticity is a well-established pathophysiologic feature of depression, little is known about disorder-associated enhanced cognitive processing. Here, we studied a novel mouse paradigm that potentially models augmented learning of adverse memories during development of a depressive-like state. We used a modification of the classic two-day protocol of a mouse Porsolt test with an additional session occurring on Day 5 following the initial exposure. Unexpectedly, floating behaviour and brain glycogen synthase kinase-3 beta (GSK-3beta) mRNA levels, a factor of synaptic plasticity as well as a marker of distress and depression, were increased during the additional swimming session that was prevented by imipramine. Observed increases of GSK-3beta mRNA in prefrontal cortex during delayed testing session correlated with individual parameters of behavioural despair that was not found in the classic Porsolt test. Repeated swim exposure was accompanied by a lower pGSK-3beta/GSK-3beta ratio. A replacement of the second or the final swim sessions with exposure to the context of testing resulted in increased GSK-3beta mRNA level similar to the effects of swimming, while exclusion of the second testing prevented these changes. Together, our findings implicate the activation of brain GSK-3beta expression in enhanced contextual conditioning of adverse memories, which is associated with an individual susceptibility to a depressive syndrome.
Databáze:	MEDLINE
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