Abnormal photoreceptor outer segment development and early retinal degeneration in kif3a mutant zebrafish.

Autor: Raghupathy RK; Department of Life Sciences, Glasgow Caledonian University, Glasgow, UK., Zhang X; Department of Life Sciences, Glasgow Caledonian University, Glasgow, UK., Alhasani RH; Department of Life Sciences, Glasgow Caledonian University, Glasgow, UK., Zhou X; Department of Life Sciences, Glasgow Caledonian University, Glasgow, UK., Mullin M; School of Life Sciences, University of Glasgow, Glasgow, UK., Reilly J; Department of Life Sciences, Glasgow Caledonian University, Glasgow, UK., Li W; School of Psychology and Neuroscience, University of St Andrews, St Andrews, UK., Liu M; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, Hubei, China., Shu X; Department of Life Sciences, Glasgow Caledonian University, Glasgow, UK.
Jazyk: angličtina
Zdroj: Cell biochemistry and function [Cell Biochem Funct] 2016 Aug; Vol. 34 (6), pp. 429-40. Date of Electronic Publication: 2016 Jul 29.
DOI: 10.1002/cbf.3205
Abstrakt: Photoreceptors are highly specialized sensory neurons that possess a modified primary cilium called the outer segment. Photoreceptor outer segment formation and maintenance require highly active protein transport via a process known as intraflagellar transport. Anterograde transport in outer segments is powered by the heterotrimeric kinesin II and coordinated by intraflagellar transport proteins. Here, we describe a new zebrafish model carrying a nonsense mutation in the kinesin II family member 3A (kif3a) gene. Kif3a mutant zebrafish exhibited curved body axes and kidney cysts. Outer segments were not formed in most parts of the mutant retina, and rhodopsin was mislocalized, suggesting KIF3A has a role in rhodopsin trafficking. Both rod and cone photoreceptors degenerated rapidly between 4 and 9 days post fertilization, and electroretinography response was not detected in 7 days post fertilization mutant larvae. Loss of KIF3A in zebrafish also resulted in an intracellular transport defect affecting anterograde but not retrograde transport of organelles. Our results indicate KIF3A plays a conserved role in photoreceptor outer segment formation and intracellular transport.
(Copyright © 2016 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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