Olig2 regulates Purkinje cell generation in the early developing mouse cerebellum.

Autor:	Ju J; West China Developmental &Stem Cell Institute, Department of Obstetric &Gynecologic and Pediatric, Key Laboratory of Obstetric &Gynecologic and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second University Hospital, Sichuan UniversityChengdu, 610041, P.R. China.; School of Life Science, Sichuan University, Chengdu 610041, P.R. China., Liu Q; West China Developmental &Stem Cell Institute, Department of Obstetric &Gynecologic and Pediatric, Key Laboratory of Obstetric &Gynecologic and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second University Hospital, Sichuan UniversityChengdu, 610041, P.R. China., Zhang Y; West China Developmental &Stem Cell Institute, Department of Obstetric &Gynecologic and Pediatric, Key Laboratory of Obstetric &Gynecologic and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second University Hospital, Sichuan UniversityChengdu, 610041, P.R. China.; School of Life Science, Sichuan University, Chengdu 610041, P.R. China., Liu Y; West China Developmental &Stem Cell Institute, Department of Obstetric &Gynecologic and Pediatric, Key Laboratory of Obstetric &Gynecologic and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second University Hospital, Sichuan UniversityChengdu, 610041, P.R. China.; School of Life Science, Sichuan University, Chengdu 610041, P.R. China., Jiang M; West China Developmental &Stem Cell Institute, Department of Obstetric &Gynecologic and Pediatric, Key Laboratory of Obstetric &Gynecologic and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second University Hospital, Sichuan UniversityChengdu, 610041, P.R. China., Zhang L; Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA., He X; Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA., Peng C; West China Developmental &Stem Cell Institute, Department of Obstetric &Gynecologic and Pediatric, Key Laboratory of Obstetric &Gynecologic and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second University Hospital, Sichuan UniversityChengdu, 610041, P.R. China.; School of Life Science, Sichuan University, Chengdu 610041, P.R. China., Zheng T; West China Developmental &Stem Cell Institute, Department of Obstetric &Gynecologic and Pediatric, Key Laboratory of Obstetric &Gynecologic and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second University Hospital, Sichuan UniversityChengdu, 610041, P.R. China., Lu QR; Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA., Li H; West China Developmental &Stem Cell Institute, Department of Obstetric &Gynecologic and Pediatric, Key Laboratory of Obstetric &Gynecologic and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second University Hospital, Sichuan UniversityChengdu, 610041, P.R. China.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2016 Jul 29; Vol. 6, pp. 30711. Date of Electronic Publication: 2016 Jul 29.
DOI:	10.1038/srep30711
Abstrakt:	The oligodendrocyte transcription factor Olig2 plays a crucial role in the neurogenesis of both spinal cord and brain. In the cerebellum, deletion of both Olig2 and Olig1 results in impaired genesis of Purkinje cells (PCs) and Pax2(+) interneurons. Here, we perform an independent study to show that Olig2 protein is transiently expressed in the cerebellar ventricular zone (VZ) during a period when PCs are specified. Further analyses demonstrate that Olig2 is expressed in both cerebellar VZ progenitors and early-born neurons. In addition, unlike in the ganglionic eminence of the embryonic forebrain where Olig2 is mostly expressed in proliferating progenitors, Olig2(+) cells in the cerebellar VZ are in the process of leaving the cell cycle and differentiating into postmitotic neurons. Functionally, deletion of Olig2 alone results in a preferential reduction of PCs in the cerebellum, which is likely mediated by decreased neuronal generation from their cerebellar VZ progenitors. Furthermore, our long-term lineage tracing experiments show that cerebellar Olig gene-expressing progenitors produce PCs but rarely Pax2(+) interneurons in the developing cerebellum, which opposes the "temporal identity transition" model of the cerebellar VZ progenitors stating that majority of Pax2(+) interneuron progenitors are transitioned from Olig2(+) PC progenitors.
Databáze:	MEDLINE
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