| Autor: |
Tanackovic V; Department of Plant and Environmental Sciences, University of Copenhagen, Denmark., Rydahl MG; Department of Plant and Environmental Sciences, University of Copenhagen, Denmark., Pedersen HL; Department of Plant and Environmental Sciences, University of Copenhagen, Denmark., Motawia MS; Department of Plant and Environmental Sciences, University of Copenhagen, Denmark., Shaik SS; Department of Plant and Environmental Sciences, University of Copenhagen, Denmark., Mikkelsen MD; Department of Plant and Environmental Sciences, University of Copenhagen, Denmark., Krunic SL; Department of Plant and Environmental Sciences, University of Copenhagen, Denmark., Fangel JU; Department of Plant and Environmental Sciences, University of Copenhagen, Denmark., Willats WG; Department of Plant and Environmental Sciences, University of Copenhagen, Denmark., Blennow A; Department of Plant and Environmental Sciences, University of Copenhagen, Denmark. |
| Abstrakt: |
In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches. |
