Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical study.

Autor:	Kumar NK; Department of Pedodontics, C.K.S. Teja Institute of Dental Sciences, Tirupati, Andhra Pradesh, India., Reddy VK; Department of Pedodontics, C.K.S. Teja Institute of Dental Sciences, Tirupati, Andhra Pradesh, India., Padakandla P; Department of Pedodontics, C.K.S. Teja Institute of Dental Sciences, Tirupati, Andhra Pradesh, India., Togaru H; Department of Pedodontics, C.K.S. Teja Institute of Dental Sciences, Tirupati, Andhra Pradesh, India., Kalagatla S; Department of Pedodontics, C.K.S. Teja Institute of Dental Sciences, Tirupati, Andhra Pradesh, India., Chandra SN; Department of Pedodontics, C.K.S. Teja Institute of Dental Sciences, Tirupati, Andhra Pradesh, India.
Jazyk:	angličtina
Zdroj:	Journal of the Indian Society of Pedodontics and Preventive Dentistry [J Indian Soc Pedod Prev Dent] 2016 Jul-Sep; Vol. 34 (3), pp. 273-9.
DOI:	10.4103/0970-4388.186754
Abstrakt:	Aims and Objectives: The study was conducted to detect the presence of macrophage inflammatory protein-1α (MIP-1α) and MIP-1β and estimate their levels in gingival crevicular fluid (GCF) in children with dental caries and stainless steel crowns. Materials and Methods: A total of 80 children with primary dentition were selected and categorized into four groups with twenty in each group; Group 1 - healthy subjects, Group 2 - dental caries, Group 3 - dental caries involving the pulp, and Group 4 - stainless steel crowns. GCF samples were collected by an extra-crevicular method with microcapillary pipettes. The GCF samples were quantified by ELISA and the levels of MIP-1α and MIP-1β were determined. Results: MIP-1α and MIP-1β were detected in all the samples. Highest mean concentration in GCF was obtained for Group 3 followed by Groups 2 and 4 while the lowest concentration was seen in Group 1. This suggests that MIP-1α and MIP-1β levels in GCF increased proportionately with the inflammation. Conclusions: GCF serves as a noninvasive diagnostic fluid to measure biomarkers released during dental caries initiation and progression. MIP-1α and MIP-1β chemokines can be considered as novel biomarkers, in biological mechanism underlying the pathogenesis and inflammation in children with dental caries and stainless steel crowns.
Databáze:	MEDLINE
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