[Molecular serological characteristics of weak D antigen types of the Rhesus system].

Autor:	Golovkina LL; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Stremoukhova AG; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Pushkina TD; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Kalandarov RS; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Atroshchenko GV; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Vasilyeva MN; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Surin VL; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Salomashkina VV; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Pshenichnikova OS; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Miterev GY; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Parovichnikova EN; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia., Savchenko VG; National Research Centre for Hematology, Ministry of Health of Russia, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Terapevticheskii arkhiv [Ter Arkh] 2016; Vol. 88 (7), pp. 78-83.
DOI:	10.17116/terarkh201688778-83
Abstrakt:	Aim: to estimate the spread of weak D antigen types of the Rhesus system in the citizens of the Russian Federation and a possibility of serologically identifying these types. Subjects and Methods: The red blood cells and DNA of people with weakened expression of D antigen were investigated using erythrocyte agglutination reaction in salt medium (2 methods); agglutination reaction in the gel columns containing IgM + IgG anti-D antibodies, indirect antiglobulin test with IgG anti-D antibodies (2 methods); polymerase chain reaction to establish the type of weak D. Results: A rhesus phenotype was determined in 5100 people in 2014-2015. The weakened agglutinable properties of red blood cells were detected in 102 (2%) examinees. 63 examinees underwent genotyping to identify the variants of the weak D antigen, which identified 6 weak D types. There were the most common weak D types 3 (n=31 (49.2%)) and weak D type 1 (n=18 (28.6%)), including weak D type 1.1 in one (1.6%) case. The other 4 weak D antigen types were as follows: weak D type 2 (14.3% (n=9)), weak D type 15 (4.8% (n=3)), weak D type 4.2 (DAR) (1.6% (n=1)) and weak D type 6 (1.6% (n=1)). The antiglobulin test in the gel column containing antiglobulin serum was the most sensitive serological assay to identify the weak D antigen. Only a molecular test could establish weak D type 15 in 2 samples of red blood cells with Ccdee and ccdEe phenotypes. Conclusion: The weak D antigen could be serologically identified in 96.8% of cases. When testing for weak D, particular attention should be given to people with the D-negative phenotype who had the C or E antigens. Our investigations conducted for the first time in Russia will be able to improve the immunological safety of red blood cell-containing medium transfusions for patients.
Databáze:	MEDLINE
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