Isolation and characterization of Helicobacter pylori recovered from gastric biopsies under anaerobic conditions.

Autor: Perez-Perez GI; Department of Medicine, NYU Langone Medical Center, New York, NY; Department Microbiology, NYU Langone Medical Center, New York, NY. Electronic address: Perezg02@med.nyu.edu., Van TN; Buu Dien Hospital, Hanoi, Vietnam., Thu Huong D; Institute of Biotechnology, Vietnam Academy of Sciences and Technology., Zhan G; Department of Medicine, NYU Langone Medical Center, New York, NY., Nguyet Anh D; E Hospital, Hanoi, Vietnam., Nguyet NT; Institute of Biotechnology, Vietnam Academy of Sciences and Technology., Thi LT; Institute of Biotechnology, Vietnam Academy of Sciences and Technology., Van Thinh N; Institute of Biotechnology, Vietnam Academy of Sciences and Technology., Hong-Hanh NT; Institute of Biotechnology, Vietnam Academy of Sciences and Technology; Research and Development Center for Biotechnology CBT, VUSTA.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2016 Oct; Vol. 86 (2), pp. 136-40. Date of Electronic Publication: 2016 Jul 12.
DOI: 10.1016/j.diagmicrobio.2016.07.009
Abstrakt: Background and Aim: Helicobacter pylori can survive long incubation periods under anaerobic conditions, and should be possible to isolate under anaerobic conditions. Our aim was to isolate H. pylori in anaerobic conditions, from gastric biopsies of H. pylori infected patients.
Methods: We enrolled 27 patients with bleeding (erosive) gastritis (mean age 36.3 years, 55.6% male) from Hanoi, Vietnam. H. pylori status was confirmed by qPCR.
Results: H. pylori were recovered under anaerobic and micro-aerobic conditions from gastric biopsies in 16 patients. Anaerobic conditions yielded significantly higher H. pylori recovery rates than micro-aerobic conditions (81.3% vs. 31.3%, P= 0.01). H. pylori isolates were characterized by PCR for specific virulence markers and the genotypes were similar to those previously described in this region of the world.
Conclusions: H. pylori can be isolated under anaerobic conditions. These findings may provide new insight into the physiology of this human pathogen and help to identify the route of H. pylori transmission.
Competing Interests: All the authors declared that they do not have any conflict of interest.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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