Effect of tranilast in comparison with beclomethasone in chronic murine model of asthma.

Autor: Nader MA; a Department of Pharmacology & Toxicology , College of Pharmacy, Taibah University , El-Madinah El-Munawarah , Saudi Arabia.; b Department of Pharmacology & Toxicology, Faculty of Pharmacy , Mansoura University , Mansoura , Egypt., Gameil N; b Department of Pharmacology & Toxicology, Faculty of Pharmacy , Mansoura University , Mansoura , Egypt., Abdelaziz RR; b Department of Pharmacology & Toxicology, Faculty of Pharmacy , Mansoura University , Mansoura , Egypt., Zalata KR; c Department of Clinical Pathology, Faculty of Medicine , Mansoura University , Mansoura , Egypt., Osman A; d Department of Pediatrics, Faculty of Medicine , Mansoura University , Mansoura , Egypt., Zedan MM; d Department of Pediatrics, Faculty of Medicine , Mansoura University , Mansoura , Egypt., Abo-Elkheir N; c Department of Clinical Pathology, Faculty of Medicine , Mansoura University , Mansoura , Egypt., Elsiddig AA; e Department of Pathology, Faculty of Medicine , Taibah University , El-Madinah El-Munawarah , Saudi Arabia., Zedan M; d Department of Pediatrics, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
Jazyk: angličtina
Zdroj: Experimental lung research [Exp Lung Res] 2016 Aug; Vol. 42 (6), pp. 296-306. Date of Electronic Publication: 2016 Jul 22.
DOI: 10.1080/01902148.2016.1207727
Abstrakt: Aim of the Study: The current investigation was taken to scrutinize the action of tranilast on the airway remodeling in chronic asthma in mice.
Materials and Methods: Intraperitoneal injection of ovalbumin was applied to mice for sensitization and subsequent inhalation of 1% ovalbumin three times week for 10 weeks for challenge. Beclomethasone or tranilast were given daily for the 10 week challenge period. At the end of the study, lung weight index, total collagen content, bronchoalveolar lavage level of total and differential cell counts, interleukin-13, in addition to lung tissue nitrate/nitrite and transforming growth beta-1 were measured. Also, histological analysis was done.
Results: Asthmatic mice demonstrated apparent fibrotic changes. Significant airway fibrosis was demonstrated by hyperplasia of goblet cells and thickening of airway epithelium, increased content of lung collagen, lung and bronchoalveolar lavage of transforming growth factor beta-1 and interleukin-13 mutually accompanied by reduction in nitrate/nitrite generation.
Conclusions: Beclomethasone influence on airway remodeling was mediated mainly via suppression of eosinophilic recruitment into the airways and reduction of interleukin-13 cytokine levels. Whereas, tranilast effects on airway remodeling was found to be mainly mediated via its inhibitory effect on transforming growth beta-1. Both beclomethasone and tranilast influence airway remodeling by different degrees and mechanisms.
Databáze: MEDLINE
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