| Autor: |
Gilev KV; Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia., Yurkin MA; Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia., Chernyshova ES; Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia., Strokotov DI; Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State Medical University, Krasny Prospect 52, 630091, Novosibirsk, Russia., Chernyshev AV; Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia., Maltsev VP; Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia; Novosibirsk State Medical University, Krasny Prospect 52, 630091, Novosibirsk, Russia. |
| Abstrakt: |
We propose a method for characterization of mature red blood cells (RBCs) morphology, based on measurement of light-scattering patterns (LSPs) of individual RBCs with the scanning flow cytometer and on solution of the inverse light-scattering (ILS) problem for each LSP. We considered a RBC shape model, corresponding to the minimal bending energy of the membrane with isotropic elasticity, and constructed an analytical approximation, which allows rapid simulation of the shape, given the diameter and minimal and maximal thicknesses. The ILS problem was solved by the nearest-neighbor interpolation using a preliminary calculated database of 250,000 theoretical LSPs. For each RBC in blood sample we determined three abovementioned shape characteristics and refractive index, which also allows us to calculate volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration and content. |
