Vibrio cholerae laboratory infection of the adult house fly Musca domestica.

Autor: El-Bassiony GM; Department of Entomology, Faculty of Science, Cairo University, Cairo, Egypt. ghada@sci.cu.edu.eg., Luizzi V; Department of Biology, Amherst College, Amherst, MA, U.S.A., Nguyen D; Department of Biology, Amherst College, Amherst, MA, U.S.A., Stoffolano JG Jr; Department of Plant, Soil, and Insect Sciences, Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA, U.S.A., Purdy AE; Department of Biology, Amherst College, Amherst, MA, U.S.A.
Jazyk: angličtina
Zdroj: Medical and veterinary entomology [Med Vet Entomol] 2016 Dec; Vol. 30 (4), pp. 392-402. Date of Electronic Publication: 2016 Jul 22.
DOI: 10.1111/mve.12183
Abstrakt: The present study was designed to test the hypothesis that house flies may be capable of specifically harbouring ingested Vibrio cholerae in their digestive tracts. Flies were continuously fed green fluorescent protein (GFP)-labelled, non-O1/non-O139 environmental strains of V. cholerae. Bacterial burdens were quantitatively measured using plate counts and localization was directly observed using confocal microscopy. Vibrio cholerae were present in the fly alimentary canal after just 4 h, and reached a plateau of ∼10 7 colony-forming units (CFU)/fly after 5 days in those flies most tolerant of the pathogen. However, individual flies were resistant to the pathogen: one or more flies were found to carry < 180 V. cholerae CFU at each time-point examined. In flies carrying V. cholerae, the pathogen was predominantly localized to the midgut rather than the rectal space or crop. The proportion of house flies carrying V. cholerae in the midgut was dose-dependent: the continuous ingestion of a concentrated, freshly prepared dose of V. cholerae increased the likelihood that fluorescent cells would be observed. However, V. cholerae may be a transient inhabitant of the house fly. This work represents the first demonstration that V. cholerae can inhabit the house fly midgut, and provides a platform for future studies of host, pathogen and environmental mediators of the successful colonization of this disease vector.
(© 2016 The Royal Entomological Society.)
Databáze: MEDLINE
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