| Autor: |
Akhi MT; a Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.; b Faculty of Medicine, Department of Microbiology , Tabriz University of Medical Sciences , Tabriz , Iran., Khalili Y; a Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.; b Faculty of Medicine, Department of Microbiology , Tabriz University of Medical Sciences , Tabriz , Iran.; e Social Security Organization, Emam Reza Hospital , Urmia , Iran., Ghotaslou R; b Faculty of Medicine, Department of Microbiology , Tabriz University of Medical Sciences , Tabriz , Iran., Kafil HS; b Faculty of Medicine, Department of Microbiology , Tabriz University of Medical Sciences , Tabriz , Iran., Yousefi S; c Faulty of Medicine, Department of Microbiology and Virology , Urmia University of Medical Sciences , Urmia , Iran., Nagili B; d Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences , Tabriz , Iran., Goli HR; b Faculty of Medicine, Department of Microbiology , Tabriz University of Medical Sciences , Tabriz , Iran. |
| Abstrakt: |
This investigation was undertaken to compare phenotypic and molecular methods for detection of carbapenemase-producing Pseudomonas aeruginosa. A total of 245 non-duplicated isolates of P. aeruginosa were collected from hospitalized patients. Disc diffusion method was used to identify carbapenem-resistant bacteria. Three phenotypic methods, including Modified Hodge Test (MHT), Modified Carba NP (MCNP) test and Carbapenem Inactivation Method (CIM) were used for investigation of carbapenemase production. In addition, polymerase chain reaction (PCR) was used to detect carbapenemase encoding genes. Of 245 P. aeruginosa isolates investigated, 121 isolates were carbapenem-resistant. Among carbapenem-resistant isolates, 40, 39 and 35 isolates exhibited positive results using MHT, MCNP test and CIM, respectively. PCR indicated the presence of carbapenemase genes in 35 of carbapenem-resistant isolates. MHT showed low sensitivity and specificity for carbapenemase detection among P. aeruginosa isolates in comparison to PCR. CIM was most affordable and highly specific than MCNP test compared with the molecular method. |
