Effects of carrageenan on cell permeability, cytotoxicity, and cytokine gene expression in human intestinal and hepatic cell lines.

Autor: McKim JM Jr; IONTOX, LLC, Kalamazoo, MI, United States. Electronic address: jmckim@iontox.com., Baas H; IONTOX, LLC, Kalamazoo, MI, United States., Rice GP; IONTOX, LLC, Kalamazoo, MI, United States., Willoughby JA Sr; Cyprotex, Kalamazoo, MI, United States., Weiner ML; TOXpertise, LLC, Princeton, NJ, United States., Blakemore W; Celtic Colloids, Inc., Topsham, ME, United States.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2016 Oct; Vol. 96, pp. 1-10. Date of Electronic Publication: 2016 Jul 14.
DOI: 10.1016/j.fct.2016.07.006
Abstrakt: Carrageenan (CGN) is a common food additive used for its gelling and thickening properties. The present study was done to evaluate intestinal permeability, cytotoxicity, and CGN-mediated induction of proinflammatory cytokines. A standard Caco-2 absorption model showed no CGN permeability or cytotoxicity at concentrations of 100, 500, and 1000 μg/mL. In two human intestinal cell lines (HT-29 and HCT-8) CGN (0.1, 1.0, and 10.0 μg/mL) did not induce IL-8, IL-6, or MCP-1 (CCL2) or produce cellular toxicity after 24 h. The TLR4 agonist LPS produced weak induction of IL-8 in HT-29 cells and no induction in HCT-8 cells. The effects of κ-CGN (0.1, 1.0, and 10 μg/mL) on cellular oxidative stress was assessed in HT-29 cells using CM-H2DCFDA as the probe. No effect on oxidative stress was observed after 24 h. In the human (HepG2) liver cell line, ʎ-CGN (0.1, 1.0, 10.0 and 100.0 μg/mL) had no effect on the expression of IL-8, IL-6, or MCP-1 (CCL2) after 24 h. In conclusion, CGN was not absorbed, and was not cytotoxic. It did not induce oxidative stress, and did not induce proinflammatory proteins.
(Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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