| Autor: |
Otto GP; Center for Sepsis Control and Care and Clinic for Anesthesiology and Intensive Care, Jena University Hospital, Jena, Germany; German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Neuherberg, Germany., Rathkolb B; German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum Muenchen, Germany; German Research Center for Environmental Health, Neuherberg, Germany; Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universitaet Muenchen, Munich, Germany; German Center for Diabetes Research, Neuherberg, Germany. birgit.rathkolb@helmholtz-muenchen.de., Oestereicher MA; German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum Muenchen, Germany; German Research Center for Environmental Health, Neuherberg, Germany., Lengger CJ; German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum Muenchen, Germany; German Research Center for Environmental Health, Neuherberg, Germany., Moerth C; German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum Muenchen, Germany; German Research Center for Environmental Health, Neuherberg, Germany; Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universitaet Muenchen, Munich, Germany., Micklich K; German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum Muenchen, Germany; German Research Center for Environmental Health, Neuherberg, Germany; Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universitaet Muenchen, Munich, Germany., Fuchs H; German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum Muenchen, Germany; German Research Center for Environmental Health, Neuherberg, Germany., Gailus-Durner V; German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum Muenchen, Germany; German Research Center for Environmental Health, Neuherberg, Germany., Wolf E; Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universitaet Muenchen, Munich, Germany., Hrabě de Angelis M; German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum Muenchen, Germany; German Research Center for Environmental Health, Neuherberg, Germany; German Center for Diabetes Research, Neuherberg, Germany; Experimental Genetics, Center of Life and Food Sciences, Weihenstephan, Technische Universitaet Muenchen, Freising-Weihenstephan, Germany. |
| Abstrakt: |
Although various mouse inbred strains are widely used to investigate disease mechanisms and to establish new therapeutic strategies, sex-specific reference intervals for laboratory diagnostic analytes that are generated from large numbers of animals have been unavailable. In this retrospective study, we screened data from more than 12,000 mice phenotyped in the German Mouse Clinic from January 2006 through June 2014 and selected animals with the genetic background of C57BL/6J, C57BL/6N, or C3HeB/FeJ. In addition, we distinguished between the C57BL/6NTac substrain and C57BL/6N mice received from other vendors. The corresponding data sets of electrolytes (sodium, potassium, calcium, chloride, inorganic phosphate), lipids (cholesterol, triglyceride), and enzyme activities (ALT, AST, ALP, α-amylase) and urea, albumin, and total protein levels were analyzed. Significant effects of age and sex on these analytes were identified, and strain- or substrain- and sex-specific reference intervals for 90- to 135-d-old mice were calculated. In addition, we include an overview of the literature that reports clinical chemistry values for wild-type mice of different strains. Our results support researchers interpreting clinical chemistry values from various mouse mutants and corresponding wild-type controls based on the examined strains and substrains. |
