Protective effect of Gallic acid on doxorubicin-induced testicular and epididymal toxicity.

Autor: Olusoji MJ; Department of Surgery and Reproduction, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria., Oyeyemi OM; Department of Surgery and Reproduction, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria., Asenuga ER; Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, University of Benin, Benin, Nigeria., Omobowale TO; Veterinary Medicine, University of Ibadan, Ibadan, Nigeria., Ajayi OL; Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria., Oyagbemi AA; Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria.
Jazyk: angličtina
Zdroj: Andrologia [Andrologia] 2017 May; Vol. 49 (4). Date of Electronic Publication: 2016 Jul 15.
DOI: 10.1111/and.12635
Abstrakt: The effect of Gallic acid (GA) on doxorubicin (DOX)-induced testicular and epididymal toxicity was investigated in experimental rat model. The rats were randomly divided into six groups of 10 animals per group. Rats in group A received clean tap water ad libitum. Rats in group B were administered DOX intraperitoneally at 15 mg/kg on the eighth day of the experiment. Animals in groups C and D received 60 and 120 mg/kg GA orally for 7 days with 15 mg/kg DOX on the eighth day. Rats in groups E and F received 60 and 120 mg/kg GA alone orally for 7 days. The animals were sacrificed 24 hr after the last administration. DOX administration led to a significant (p < 0.05) increase in hydrogen peroxide and malondialdehyde levels with significant reduction in antioxidant enzymes and reduced glutathione levels. DOX administration also led to a significant increase in total sperm abnormalities and prolactin together with a significant decrease in testosterone levels. Immunohistochemistry revealed higher expressions of caspase 3 in the testicular tissues of rats that received DOX alone. Together, pre-treatment with GA attenuated markers of oxidative stress, reversed sperm abnormality and ameliorated the observed aberration in plasma testosterone and prolactin levels.
(© 2016 Blackwell Verlag GmbH.)
Databáze: MEDLINE
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