Effect of Fagonia arabica on thrombin induced release of t-PA and complex of PAI-1 tPA in cultured HUVE cells.

Autor:	Aloni PD; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India., Nayak AR; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India., Chaurasia SR; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India., Deopujari JY; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India., Chourasia C; Matru Sewa Sangh, Maternity Home, Kothi Road, Mahal, Nagpur 440002, Maharashtra, India., Purohit HJ; Environmental Genomics Unit, NEERI, Nehru Marg, Nagpur 440020, Maharashtra, India., Taori GM; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India., Daginawala HF; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India., Kashyap RS; Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur 440010, Maharashtra, India.
Jazyk:	angličtina
Zdroj:	Journal of traditional and complementary medicine [J Tradit Complement Med] 2015 Apr 03; Vol. 6 (3), pp. 219-23. Date of Electronic Publication: 2015 Apr 03 (Print Publication: 2016).
DOI:	10.1016/j.jtcme.2015.03.002
Abstrakt:	Fagonia arabica (FA) possesses a thrombolytic property which has been earlier reported in our laboratory. Current study was undertaken to investigate the effect of aqueous extract of FA on thrombin-induced tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) release from cultured human umbilical vein endothelial cell line (HUVE) for studying its clot lytic activity. For this, establishment of cell line model has been done by isolating the cells from human umbilical cord. Cell toxicity was evaluated using XTT assay. Estimation of t-PA and PAI-1 t-PA complex were done using ELISA technique. Thrombin treatment induces the t-PA and PAI-1 release from HUVE cell line, and FA treatment was found to antagonize the thrombin induced t-PA and PAI-1 release. Our preliminary results suggest that FA may be used as an alternative to thrombolytic drug. However, study demands further experiments using animal model of thrombosis to establish the role of FA as a novel thrombolytic drug.
Databáze:	MEDLINE
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