Origin of bimodal fluorescence enhancement factors of Chlorobaculum tepidum reaction centers on silver island films.

Autor: Maćkowski S; Optics of Hybrid Nanostructures Group, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Torun, Poland., Czechowski N; Optics of Hybrid Nanostructures Group, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Torun, Poland., Ashraf KU; Institute of Molecular, Cell & Systems Biology, Glasgow Biomedical Research Centre, University of Glasgow, UK., Szalkowski M; Optics of Hybrid Nanostructures Group, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Torun, Poland., Lokstein H; Institute of Molecular, Cell & Systems Biology, Glasgow Biomedical Research Centre, University of Glasgow, UK., Cogdell RJ; Institute of Molecular, Cell & Systems Biology, Glasgow Biomedical Research Centre, University of Glasgow, UK., Kowalska D; Optics of Hybrid Nanostructures Group, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Torun, Poland.
Jazyk: angličtina
Zdroj: FEBS letters [FEBS Lett] 2016 Aug; Vol. 590 (16), pp. 2558-65. Date of Electronic Publication: 2016 Jul 26.
DOI: 10.1002/1873-3468.12292
Abstrakt: We focus on the spectral dependence of plasmon-induced enhancement of fluorescence of Chlorobaculum tepidum reaction centers. When deposited on silver island film, they exhibit up to a 60-fold increase in fluorescence. The dependence of enhancement factors on the excitation wavelength is not correlated with the absorption spectrum of the plasmonic structure. In particular, the presence of one (or multiple) trimers of the Fenna-Matthews-Olson (FMO) protein reveals itself in bimodal distribution of enhancement factors for the excitation at 589 nm, the wavelength corresponding to bacteriochlorophyll absorption of FMO and the core of the RC. We conclude that the structure of multichromophoric complexes can substantially affect the impact of plasmonic excitations, which is important in the context of assembling functional biohybrid systems.
(© 2016 Federation of European Biochemical Societies.)
Databáze: MEDLINE
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