| Autor: |
Stessuk T; University of São Paulo, USP, São Paulo, Brazil., Puzzi MB; University of Campinas, UNICAMP, Campinas, Brazil., Chaim EA; University of Campinas, UNICAMP, Campinas, Brazil., Alves PC; University of Campinas, UNICAMP, Campinas, Brazil., de Paula EV; University of Campinas, UNICAMP, Campinas, Brazil., Forte A; CordCell Cell Therapy, São Paulo, Brazil., Izumizawa JM; University of Campinas, UNICAMP, Campinas, Brazil., Oliveira CC; University of Campinas, UNICAMP, Campinas, Brazil., Frei F; São Paulo State University, UNESP, Campus de Assis, Assis, Brazil., Ribeiro-Paes JT; São Paulo State University, UNESP, Campus de Assis, Assis, Brazil. jtrpaes@yahoo.com.br.; Department of Biological Sciences, São Paulo State University, UNESP, Av. Dom Antonio, 2100, Assis, São Paulo, 19.806-900, Brazil. jtrpaes@yahoo.com.br. |
| Abstrakt: |
The clinical use of tissue engineering associated with cell therapy is considered a new alternative therapy for the repair of chronic lesions with potential application in different medical areas, mostly in orthopedic and dermatological diseases. Platelet-rich plasma (PRP) is a rich source of growth factors and cytokines important for wound healing. Adipose-derived mesenchymal stem cells (ADSCs) have shown potential to accelerate the resolution of ulcers, to stimulate cell proliferation, and to benefit the quality of skin repair. This study aims to determine the effect of PRP and conditioned medium (CM) from ADSC on fibroblast and keratinocyte proliferation in vitro. Migration and proliferation assays were performed to evaluate the growth of fibroblasts and keratinocytes in the presence of PRP, CM, and CM + PRP. Significant proliferative stimulation was observed after 48 h of culture (p < 0.05) on mean absorbance of fibroblasts cultured with 10 and 25 % PRP, 100 % CM, and 25 % PRP + 25 % CM, if compared with control. Keratinocyte proliferation was stimulated after 48 h in cultures with 25, 50, and 100 % CM, and growth was compared with controls. The migration assay detected a significant migratory stimulus in fibroblasts cultured with 10 % PRP + 10 % CM after 48 h. These in vitro results suggest that PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds in vivo. |
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