Histological study on the protective role of vitamin B complex on the cerebellum of diabetic rat.

Autor: Eltony SA; Histology Department, Faculty of Medicine, Assiut University, Egypt. Electronic address: sohair_eltony@yahoo.com.
Jazyk: angličtina
Zdroj: Tissue & cell [Tissue Cell] 2016 Aug; Vol. 48 (4), pp. 283-96. Date of Electronic Publication: 2016 Jun 23.
DOI: 10.1016/j.tice.2016.06.009
Abstrakt: Background: Disorder in cerebellar structure was reported in diabetes mellitus. B vitamins are involved in many significant metabolic processes within the brain.
Aim of the Work: To detect the protective role of vitamin B complex on the histological structure of the cerebellum of experimentally induced diabetic rat.
Material & Methods: Eighteen adult male Wistar rats were divided into two groups. Group I: normal vehicle control (n=6). Group II: streptozotocin-induced diabetic rats (n=12), which was equally divided into two subgroups; IIA (diabetic vehicle control), IIB (diabetic vitamin B complex-treated): streptozotocin-induced diabetic rats treated with vitamin B complex (1mg/kg/day) for 6 weeks. Specimens from the cerebellum were processed for light and electron microscopy.
Results: In vitamin B complex treated group, the histological changes in Purkinje cells, astrocytes and oligodendrocytes were improved compared with the diabetic non-treated group. The white matter revealed intact myelinated axons. Inducible nitric oxide synthase (iNOS) and caspase-3 expression reduced. Glial fibrillary acidic protein (GFAP) expression revealed less activated astroglia. The number of Purkinje cells/mm(2) significantly increased. While, the number of GFAP positive astrocytes/mm(2) significantly decreased. In addition, the blood glucose level was reduced.
Conclusion: Vitamin B complex protected the cerebellum from the histological changes which occurred in STZ- induced diabetic rats.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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