| Autor: |
Lara-Oya A; Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain., Rodríguez-Granger JM; Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain., Liébana-Martos MDC; Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain., Mendoza-López P; Vircell S.L. Research, Calle Avicena, 18016 Granada, Spain., Sampedro-Martínez A; Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain., Cobo F; Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain., Gutierrez-Fernández J; Department of Microbiology, School of Medicine, University of Granada, Avenida de Madrid, 11, 18012 Granada, Spain., Martínez-Lirola MJ; Department of Microbiology, Torrecardenas Hospital, Paraje Torrecárdenas s/n, 04009 Almeria, Spain., Navarro-Marí JM; Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain. |
| Abstrakt: |
We present the first evaluation of a novel molecular assay, the Speed-Oligo Mycobacterium tuberculosis complex (SO-MTBC), which is based on PCR combined with a dipstick for the differentiation of M. tuberculosis complex (MTBC) members. The results of this assay were compared with findings obtained using the Genotype MTBC assay. In this study, 189 strains of MTBC isolates from 2011 to 2014 were evaluated to determine the MTBC species. Most (174, 92 %) of the strains were identified as M. tuberculosissensu stricto, 7 (3.7 %) as Mycobacteriumbovis, 5 (2.6 %) as M. bovis bacillus Calmette-Guérin, 2 (1.1 %) as Mycobacteriumafricanum and 1 (0.5 %) as Mycobacteriumcaprae; no strains belonged to Mycobacteriummicroti and Mycobacteriumcanettii subsp. The concordance κ coefficient obtained was 0.96 with the results of the Genotype MTBC assay. SO-MTBC may represent a fast and easy-to-use alternative for differentiating among MTBC subspecies in laboratories with standard equipment. |
