Singlet oxygen production by combining erythrosine and halogen light for photodynamic inactivation of Streptococcus mutans.

Autor: Fracalossi C; Dentistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, Cep 87020-900, Brazil., Nagata JY; Endodontic Department, Federal University of Sergipe, Avenida Marechal Rondon, S/n - Jardim Rosa Elze, Largarto, São Cristóvão, SE, Cep 49100-000, Brazil. Electronic address: jynagata@gmail.com., Pellosi DS; Chemistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, Cep 87020-900, Brazil., Terada RS; Dentistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, Cep 87020-900, Brazil., Hioka N; Chemistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, Cep 87020-900, Brazil. Electronic address: nhioka@uem.br., Baesso ML; Physics Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, Cep 87020-900, Brazil. Electronic address: mlbaesso@gmail.com., Sato F; Physics Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, Cep 87020-900, Brazil., Rosalen PL; Department of Physiological Sciences, School of Dentistry of Piracicaba, University of Campinas, Avenida Limeira, 901 - Bairro Areião, Piracicaba, SP, Cep 13414-903, Brazil. Electronic address: rosalen@fop.unicamp.br., Caetano W; Chemistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, Cep 87020-900, Brazil., Fujimaki M; Dentistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, Cep 87020-900, Brazil. Electronic address: mfujimaki@uem.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2016 Sep; Vol. 15, pp. 127-32. Date of Electronic Publication: 2016 Jun 30.
DOI: 10.1016/j.pdpdt.2016.06.011
Abstrakt: Background: Photodynamic inactivation of microorganisms is based on a photosensitizing substance which, in the presence of light and molecular oxygen, produces singlet oxygen, a toxic agent to microorganisms and tumor cells. This study aimed to evaluate singlet oxygen quantum yield of erythrosine solutions illuminated with a halogen light source in comparison to a LED array (control), and the photodynamic effect of erythrosine dye in association with the halogen light source on Streptococcus mutans.
Methods: Singlet oxygen quantum yield of erythrosine solutions was quantified using uric acid as a chemical-probe in an aqueous solution. The in vitro effect of the photodynamic antimicrobial activity of erythrosine in association with the halogen photopolimerizing light on Streptococcus mutans (UA 159) was assessed during one minute. Bacterial cultures treated with erythrosine alone served as negative control.
Results: Singlet oxygen with 24% and 2.8% degradation of uric acid in one minute and a quantum yield of 0.59 and 0.63 was obtained for the erythrosine samples illuminated with the halogen light and the LED array, respectively. The bacterial cultures with erythrosine illuminated with the halogen light presented a decreased number of CFU mL(-1) in comparison with the negative control, with minimal inhibitory concentrations between 0.312 and 0.156mgmL(-1).
Conclusions: The photodynamic response of erythrosine induced by the halogen light was capable of killing S. mutans. Clinical trials should be conducted to better ascertain the use of erythrosine in association with halogen light source for the treatment of dental caries.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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