Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant Staphylococcus aureus isolates.

Autor: Argudín MA; Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Max-Dohrn-Straße 8-10, Berlin, Germany; Department of Bacterial Diseases, Veterinary and Agrochemical Research Centre (CODA-CERVA), Brussels, Groeselenbergstraat 99, B-1180 Ukkel, Belgium., Lauzat B; Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Max-Dohrn-Straße 8-10, Berlin, Germany., Kraushaar B; Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Max-Dohrn-Straße 8-10, Berlin, Germany., Alba P; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri' (IZSLT), Via Appia Nuova 1411, 00178, Rome, Italy., Agerso Y; Technical University of Denmark (DTU), National Food Institute, Research group for Genomic Epidemiology, Søltofts Plads Building 221, 2800 Lyngby, Denmark., Cavaco L; Technical University of Denmark (DTU), National Food Institute, Research group for Genomic Epidemiology, Søltofts Plads Building 221, 2800 Lyngby, Denmark., Butaye P; Department of Biomedical Sciences, Ross University, P.O. Box 334, Basseterre, St Kitts, West Indies; Department of Pathology, Bacteriology, and Avian Diseases, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium., Porrero MC; Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense Madrid (UCM), Madrid, Spain., Battisti A; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri' (IZSLT), Via Appia Nuova 1411, 00178, Rome, Italy., Tenhagen BA; Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Max-Dohrn-Straße 8-10, Berlin, Germany., Fetsch A; Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Max-Dohrn-Straße 8-10, Berlin, Germany., Guerra B; Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Max-Dohrn-Straße 8-10, Berlin, Germany. Electronic address: beatriz.guerra@efsa.europa.eu.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2016 Aug 15; Vol. 191, pp. 88-95. Date of Electronic Publication: 2016 Jun 09.
DOI: 10.1016/j.vetmic.2016.06.004
Abstrakt: Livestock associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has emerged in animal production worldwide. Most LA-MRSA in Europe belong to the clonal complex (CC) 398. The reason for the LA-MRSA emergence is not fully understood. Besides antimicrobial agents used for therapy, other substances with antimicrobial activity applied in animal feed, including metal-containing compounds might contribute to their selection. Some of these genes have been found in various novel SCCmec cassettes. The aim of this study was to assess the occurrence of metal-resistance genes among a LA-S. aureus collection [n=554, including 542 MRSA and 12 methicillin-susceptible S. aureus (MSSA)] isolated from livestock and food thereof. Most LA-MRSA isolates (76%) carried at least one metal-resistance gene. Among the LA-MRSA CC398 isolates (n=456), 4.8%, 0.2%, 24.3% and 71.5% were positive for arsA (arsenic compounds), cadD (cadmium), copB (copper) and czrC (zinc/cadmium) resistance genes, respectively. In contrast, among the LA-MRSA non-CC398 isolates (n=86), 1.2%, 18.6% and 16.3% were positive for the cadD, copB and czrC genes, respectively, and none were positive for arsA. Of the LA-MRSA CC398 isolates, 72% carried one metal-resistance gene, and the remaining harboured two or more in different combinations. Differences between LA-MRSA CC398 and non-CC398 were statistically significant for arsA and czrC. The czrC gene was almost exclusively found (98%) in the presence of SCCmec V in both CC398 and non-CC398 LA-MRSA isolates from different sources. Regarding the LA-MSSA isolates (n=12), some (n=4) were also positive for metal-resistance genes. This study shows that genes potentially conferring metal-resistance are frequently present in LA-MRSA.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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