Identifying Centromeric RNAs Involved in Histone Dynamics In Vivo.
| Autor: | Quénet D; Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States. Electronic address: delphine.quenet@med.uvm.edu., Sturgill D; Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States., Dalal Y; Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States. Electronic address: dalaly@mail.nih.gov. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in enzymology [Methods Enzymol] 2016; Vol. 573, pp. 445-66. Date of Electronic Publication: 2016 Mar 24. |
| DOI: | 10.1016/bs.mie.2016.01.010 |
| Abstrakt: | Over the last decade, the long accepted dogma that heterochromatin is silent has been challenged by increasing evidence of active transcription in these apocryphally annotated quiescent regions of the genome. The recent discovery of noncoding RNAs (ncRNAs) originating from, or localizing to, centromeres, pericentromeres, and telomeres (ie, constitutive heterochromatin) suggest a potential role for ncRNAs in genome integrity. This new paradigm suggests that ncRNAs may recruit chromatin-binding factors, stabilize the higher order folded state of the chromatin fiber, and participate in regulation of processes such as transcription-mediated nucleosome assembly. Thus, identifying, purifying, and elucidating the function of ncRNAs has the potential to provide key insights into genome organization and is currently a topic of intense experimental investigation. (© 2016 Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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