Performance of Alere™ immunochromathographic test for the diagnosis of canine visceral leishmaniasis.

Autor: Souza Filho JA; Brazilian National Reference Laboratory for Diagnosis of Visceral Leishmaniasis, Serviço de Doenças Parasitárias, Instituto Octavio Magalhães, Fundação Ezequiel Dias (FUNED), Belo Horizonte-MG, Brazil. Electronic address: job.filho@funed.mg.gov.br., Barbosa JR; Brazilian National Reference Laboratory for Diagnosis of Visceral Leishmaniasis, Serviço de Doenças Parasitárias, Instituto Octavio Magalhães, Fundação Ezequiel Dias (FUNED), Belo Horizonte-MG, Brazil., Figueiredo FB; Instituto Nacional de Infectologia Evandro Chagas/Fundação Oswaldo Cruz (FioCruz), Rio de Janeiro-RJ, Brazil., Mendes AA Júnior; Instituto Nacional de Infectologia Evandro Chagas/Fundação Oswaldo Cruz (FioCruz), Rio de Janeiro-RJ, Brazil., Silva SR; Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, Minas Gerais, Brazil., Coelho GL; Escola de Medicina, Universidade Federal de Ouro Preto, Ouro Preto-MG, Brazil., Marcelino AP; Brazilian National Reference Laboratory for Diagnosis of Visceral Leishmaniasis, Serviço de Doenças Parasitárias, Instituto Octavio Magalhães, Fundação Ezequiel Dias (FUNED), Belo Horizonte-MG, Brazil. Electronic address: andreza.marcelino@funed.mg.gov.br.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2016 Jul 30; Vol. 225, pp. 114-6. Date of Electronic Publication: 2016 Jun 09.
DOI: 10.1016/j.vetpar.2016.06.011
Abstrakt: The diagnosis and control of canine visceral leishmaniasis (CVL) has fundamental importance in the control of human visceral leishmaniasis. In this context, the immunochromathographic test (ICT) has emerged as a valuable diagnostic tool. Currently, in Brazil, the algorithm for the diagnosis of CVL uses Dual Path Platform (DPP) ICT as a screening test and ELISA as a confirmatory test. However, the DPP(®) ICT has been facing a problem of insufficient production to meet the national demand. Here, the Alere™ canine visceral leishmaniasis ICT was tested with serum samples from 159 dogs, including symptomatic, asymptomatic and healthy dogs, characterized by DPP(®) ICT and ELISA (panel A), or ELISA and Indirect Immunofluorescence Assay (IFA) (panel B), and parasitological examination as gold standard. In this study, the Alere™ ICT obtained 0.974 sensitivity for panel A and 0.854 sensitivity for panel B. The specificity was 1.0 for both panels. Thus, the Alere™ ICT has potential to be used for the serological diagnosis of CVL.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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