Guiding synchrotron X-ray diffraction by multimodal video-rate protein crystal imaging.

Autor: Newman JA; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA., Zhang S; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA., Sullivan SZ; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA., Dow XY; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA., Becker M; GM/CA@APS, X-Ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA., Sheedlo MJ; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA., Stepanov S; GM/CA@APS, X-Ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA., Carlsen MS; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA., Everly RM; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA., Das C; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA., Fischetti RF; GM/CA@APS, X-Ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA., Simpson GJ; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47906, USA.
Jazyk: angličtina
Zdroj: Journal of synchrotron radiation [J Synchrotron Radiat] 2016 Jul; Vol. 23 (Pt 4), pp. 959-65. Date of Electronic Publication: 2016 May 16.
DOI: 10.1107/S1600577516005919
Abstrakt: Synchronous digitization, in which an optical sensor is probed synchronously with the firing of an ultrafast laser, was integrated into an optical imaging station for macromolecular crystal positioning prior to synchrotron X-ray diffraction. Using the synchronous digitization instrument, second-harmonic generation, two-photon-excited fluorescence and bright field by laser transmittance were all acquired simultaneously with perfect image registry at up to video-rate (15 frames s(-1)). A simple change in the incident wavelength enabled simultaneous imaging by two-photon-excited ultraviolet fluorescence, one-photon-excited visible fluorescence and laser transmittance. Development of an analytical model for the signal-to-noise enhancement afforded by synchronous digitization suggests a 15.6-fold improvement over previous photon-counting techniques. This improvement in turn allowed acquisition on nearly an order of magnitude more pixels than the preceding generation of instrumentation and reductions of well over an order of magnitude in image acquisition times. These improvements have allowed detection of protein crystals on the order of 1 µm in thickness under cryogenic conditions in the beamline. These capabilities are well suited to support serial crystallography of crystals approaching 1 µm or less in dimension.
Databáze: MEDLINE
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