Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells.

Autor: Mota Ferreira L; a Programa de Pós-graduação em Ciências Farmacêuticas , Centro de Ciências da Saúde, Universidade Federal de Santa Maria , Santa Maria , Brazil., Gehrcke M; a Programa de Pós-graduação em Ciências Farmacêuticas , Centro de Ciências da Saúde, Universidade Federal de Santa Maria , Santa Maria , Brazil., Ferrari Cervi V; a Programa de Pós-graduação em Ciências Farmacêuticas , Centro de Ciências da Saúde, Universidade Federal de Santa Maria , Santa Maria , Brazil., Eliete Rodrigues Bitencourt P; a Programa de Pós-graduação em Ciências Farmacêuticas , Centro de Ciências da Saúde, Universidade Federal de Santa Maria , Santa Maria , Brazil., Ferreira da Silveira E; b Programa de Pós-Graduação em Bioquímica e Bioprospecção , Centro de Ciências Químicas, Farmacêuticas e de Alimentos , Pelotas , Brazil., Hofstatter Azambuja J; b Programa de Pós-Graduação em Bioquímica e Bioprospecção , Centro de Ciências Químicas, Farmacêuticas e de Alimentos , Pelotas , Brazil., Prates Ramos A; c Curso de Biomedicina , Centro Universitário Franciscano , Santa Maria , Brazil., Nascimento K; c Curso de Biomedicina , Centro Universitário Franciscano , Santa Maria , Brazil., Beatriz Moretto M; a Programa de Pós-graduação em Ciências Farmacêuticas , Centro de Ciências da Saúde, Universidade Federal de Santa Maria , Santa Maria , Brazil., Braganhol E; b Programa de Pós-Graduação em Bioquímica e Bioprospecção , Centro de Ciências Químicas, Farmacêuticas e de Alimentos , Pelotas , Brazil., Rorato Sagrillo M; c Curso de Biomedicina , Centro Universitário Franciscano , Santa Maria , Brazil., Cruz L; a Programa de Pós-graduação em Ciências Farmacêuticas , Centro de Ciências da Saúde, Universidade Federal de Santa Maria , Santa Maria , Brazil.
Jazyk: angličtina
Zdroj: Pharmaceutical biology [Pharm Biol] 2016 Dec; Vol. 54 (12), pp. 2968-2977. Date of Electronic Publication: 2016 Jun 29.
DOI: 10.1080/13880209.2016.1199039
Abstrakt: Context: Glioma is a malignant brain tumor with rapid proliferation, infiltrative growth, poor prognosis and it is chemoresistent. Pomegranate seed oil (PSO) has antioxidant, anti-inflammatory and antitumor properties. This study showed the optimization of PSO nanoemulsions (NEs) as an alternative for glioma treatment.
Objective: The study aimed to evaluate PSO NEs cytotoxicity on human blood cells and antiglioma effects against C6 cells.
Materials and Methods: NEs were prepared by the spontaneous emulsification method, using PSO at 1.5 and 3.0%, and were evaluated regarding their physical stability and antioxidant activity. Toxicity evaluations in human blood cells were performed in terms of cell viability, genotoxicity, lipid peroxidation, protein carbonylation, catalase activity and hemolysis at 0.1, 0.25 and 0.5 mg/mL PSO, after a 72-h incubation period. In vitro antitumor effect was determined against glioma cells after 24 and 48 h, and astrocytes were used as a non-transformed cell model.
Results: Formulations presented droplet size below 250 nm, low polydispersity index, negative zeta potential and pH in the acid range. NEs and PSO had scavenging capacity around 30% and promoted a proliferative effect in mononuclear cells, increasing about 50% cell viability. No genotoxic and oxidative damage was observed in lipid peroxidation, protein carbonylation and catalase activity evaluations for NEs. Hemolysis study showed a hemolytic effect at high concentrations. Moreover, formulations reduced only tumor cell viability to 47%, approximately.
Discussion and Conclusion: Formulations are adequate and safe for intravenous administration. Besides, in vitro antitumor activity indicates that NEs are promising for glioma treatment.
Databáze: MEDLINE
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