Proteomic Analysis of Exosomes and Exosome-Free Conditioned Media From Human Osteosarcoma Cell Lines Reveals Secretion of Proteins Related to Tumor Progression.

Autor: Jerez S; Millennium Institute on Immunology and Immunotherapy, University of Chile, Santiago, Chile.; Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile., Araya H; Millennium Institute on Immunology and Immunotherapy, University of Chile, Santiago, Chile.; Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile., Thaler R; Departments of Orthopedic Surgery & Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street S.W., MSB 3-69, Rochester, Minnesota 55905., Charlesworth MC; Mayo Proteomics Research Center, Mayo Clinic, Rochester, Minnesota 55905., López-Solís R; Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile., Kalergis AM; Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.; Departamento de Endocrinología, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile., Céspedes PF; Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile., Dudakovic A; Departments of Orthopedic Surgery & Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street S.W., MSB 3-69, Rochester, Minnesota 55905., Stein GS; Department of Biochemistry, University of Vermont College of Medicine, 89 Beaumont Avenue, Burlington, Vermont 05405., van Wijnen AJ; Departments of Orthopedic Surgery & Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street S.W., MSB 3-69, Rochester, Minnesota 55905., Galindo M; Millennium Institute on Immunology and Immunotherapy, University of Chile, Santiago, Chile.; Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
Jazyk: angličtina
Zdroj: Journal of cellular biochemistry [J Cell Biochem] 2017 Feb; Vol. 118 (2), pp. 351-360. Date of Electronic Publication: 2016 Aug 15.
DOI: 10.1002/jcb.25642
Abstrakt: Osteosarcomas are the most prevalent bone tumors in pediatric patients, but can also occur later in life. Bone tumors have the potential to metastasize to lung and occasionally other vital organs. To understand how osteosarcoma cells interact with their micro-environment to support bone tumor progression and metastasis, we analyzed secreted proteins and exosomes from three human osteosarcoma cell lines. Exosome isolation was validated by transmission electron microscopy (TEM) and immuno-blotting for characteristic biomarkers (CD63, CD9, and CD81). Exosomal and soluble proteins (less than 100 kDa) were identified by mass spectrometry analysis using nanoLC-MS/MS and classified by functional gene ontology clustering. We identified a secretome set of >3,000 proteins for both fractions, and detected proteins that are either common or unique among the three osteosarcoma cell lines. Protein ontology comparison of proteomes from exosomes and exosome-free fractions revealed differences in the enrichment of functional categories associated with different biological processes, including those related to tumor progression (i.e., angiogenesis, cell adhesion, and cell migration). The secretome characteristics of osteosarcoma cells are consistent with the pathological properties of tumor cells with metastatic potential. J. Cell. Biochem. 118: 351-360, 2017. © 2016 Wiley Periodicals, Inc.
(© 2016 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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