The effects of genistein supplementation on fructose induced insulin resistance, oxidative stress and inflammation.

Autor: Incir S; Koc University Hospital, Clinical Laboratory, Istanbul, Turkey., Bolayirli IM; Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey. Electronic address: mrtbola@yahoo.com., Inan O; Experimental Animal Research Center, Mehmet Akif State Hospital, Halkalı, Istanbul, Turkey., Aydın MS; Medical Faculty of Bezmialem Vakif University, Department of Histology and Embryology, Istanbul, Turkey., Bilgin IA; Maslak Acibadem Hospital, Department of General Surgery, Istanbul, Turkey., Sayan I; Marmara University, School of Medicine, Intensive Care Unit, Turkey., Esrefoglu M; Medical Faculty of Bezmialem Vakif University, Department of Histology and Embryology, Istanbul, Turkey., Seven A; Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2016 Aug 01; Vol. 158, pp. 57-62. Date of Electronic Publication: 2016 Jun 24.
DOI: 10.1016/j.lfs.2016.06.014
Abstrakt: Aims: This experimental study was designed to investigate the effects of 10weeks genistein administration on oxidative stress and inflammation in serum and liver of rats fed with fructose.
Main Methods: 6-8weeks old, 40 male Sprague-Dawley rats were included. Group 1 (control) was fed with standard chow food and 100μl/kg/day/rat dimethyl sulfoxide (DMSO) administered subcutaneously; group 2 (genistein) with standard chow food and 0.25mg/kg/day/rat genistein; group 3 (fructose) with standard chow food and drinking water 20% fructose, group 4 (fructose+genistein) with standard chow food, drinking water with 20% fructose and 0.25mg/kg/day/rat genistein. TNF-α, IL-6, visfatin as inflammatory markers and 8-isoprostane as a oxidative stress marker were measured by ELISA, glucose, triglyceride, total cholesterol, LDL-cholesterol and HDL-cholesterol by enzymatic colorimetric method, AST and ALT by kinetic UV method.
Key Findings: Significantly high 8-isoprostane levels in serum (p<0.001) and liver (p<0.05) in group 3 compared to control group indicate that presence of oxidative stress. Significantly high TNF-α and IL-6 levels in serum (p<0.05) and liver (p<0.01) and visfatin levels in serum (p<0.001) of group 3 indicate inflammation accompanying insulin resistance and oxidative stress. Genistein administration to fructose group causes a significant decrease in HOMA-IR (p<0.001) and LDLC (p<0.05) level. Significantly lower serum 8-isoprostane (p<0.01) level indicates the antioxidant effect of genistein and significantly lower liver TNF-α (p<0.01), serum, liver IL-6(p<0.01) and serum visfatin (p<0.01) levels reflect the antiinflammatory effects of genistein.
Significance: Genistein administration to rats fed with fructose causes an ameliorating effect on HOMA-IR values and lipid status markers in addition to its antioxidant and antiinflammatory effects.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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