Ovine trophoblasts express cathelicidin host defence peptide in response to infection.

Autor: Coyle C; School of Life, Sport and Social Sciences, Edinburgh Napier University, Sighthill Campus, Edinburgh EH11 4BN, United Kingdom., Wheelhouse N; Moredun Research Institute, Pentlands Science Park, Edinburgh, Midlothian, EH26 0PZ, United Kingdom., Jacques M; School of Life, Sport and Social Sciences, Edinburgh Napier University, Sighthill Campus, Edinburgh EH11 4BN, United Kingdom., Longbottom D; Moredun Research Institute, Pentlands Science Park, Edinburgh, Midlothian, EH26 0PZ, United Kingdom., Svoboda P; Biotechnology Core Facility Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta GA 30333, United States., Pohl J; Biotechnology Core Facility Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta GA 30333, United States., Duncan WC; MRC Centre for Reproductive Health, The Queens Medical Research Institute, The University of Edinburgh, Edinburgh EH16 4TJ, United Kingdom., Rae MT; School of Life, Sport and Social Sciences, Edinburgh Napier University, Sighthill Campus, Edinburgh EH11 4BN, United Kingdom., Barlow PG; School of Life, Sport and Social Sciences, Edinburgh Napier University, Sighthill Campus, Edinburgh EH11 4BN, United Kingdom. Electronic address: p.barlow@napier.ac.uk.
Jazyk: angličtina
Zdroj: Journal of reproductive immunology [J Reprod Immunol] 2016 Sep; Vol. 117, pp. 10-6. Date of Electronic Publication: 2016 Jun 21.
DOI: 10.1016/j.jri.2016.06.006
Abstrakt: Cationic host defence peptides (CHDP; also known as antimicrobial peptides) are key components of the immune response in the female reproductive tract. The role of the placental trophoblast in ovine host defence remains poorly understood. This study characterises expression of genes for cathelicidin and defensin peptides in primary ovine placental tissues, the ovine trophoblast cell line (AH-1) and in response to the TLR-4 ligand LPS, the abortifacient organism Waddlia chondrophila and 1α,25-dihydroxyvitamin D3. Using RT-PCR, expression of the CHDP SMAP-29, sBD-1 and sBD-2 was assessed in the AH-1 cell line in response to LPS, 1α,25-dihydroxyvitamin D3 exposure (a known stimulator of cathelicidin gene expression), or W. chondrophila infection. Expression of cathelicidin in the trophoblast compartment of the ovine placenta and in the ovine trophoblast cell line (AH-1) was also established. AH-1 cells did not upregulate expression of CHDP in response to LPS, but sBD-1 and sBD-2 expression was significantly increased in response to W. chondrophila infection. SMAP-29 expression was not altered by in vitro exposure to 1α,25-dihydroxyvitamin D3. This study demonstrates that the ovine trophoblast expresses cathelicidins, but does not upregulate expression of CHDP in response to LPS. Ovine trophoblasts are shown to differentially regulate expression of CHDP and lack a demonstrable vitamin D-mediated cathelicidin response.
(Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: