Comparative proteome analysis reveals that blood and sugar meals induce differential protein expression in Aedes aegypti female heads.

Autor: Nunes AT; Universidade Federal do Rio de Janeiro, Instituto de Química, Rio de Janeiro, Brazil., Brito NF; Universidade Federal do Rio de Janeiro, Instituto de Química, Rio de Janeiro, Brazil., Oliveira DS; Universidade Federal do Rio de Janeiro, Instituto de Química, Rio de Janeiro, Brazil., Araujo GD; Proteomics Unit, Federal University of Rio de Janeiro, Chemistry Institute, Rio de Janeiro, Brazil., Nogueira FC; Proteomics Unit, Federal University of Rio de Janeiro, Chemistry Institute, Rio de Janeiro, Brazil., Domont GB; Proteomics Unit, Federal University of Rio de Janeiro, Chemistry Institute, Rio de Janeiro, Brazil., Moreira MF; Universidade Federal do Rio de Janeiro, Instituto de Química, Rio de Janeiro, Brazil.; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, Brazil., Moreira LM; Universidade Federal de Ouro Preto, Instituto de Ciências Exatas e Biológicas, Ouro Preto, Brazil., Soares MR; Universidade Federal do Rio de Janeiro, Instituto de Química, Rio de Janeiro, Brazil., Melo AC; Universidade Federal do Rio de Janeiro, Instituto de Química, Rio de Janeiro, Brazil. anamelo@iq.ufrj.br.; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, Brazil. anamelo@iq.ufrj.br.
Jazyk: angličtina
Zdroj: Proteomics [Proteomics] 2016 Oct; Vol. 16 (19), pp. 2582-2586. Date of Electronic Publication: 2016 Aug 05.
DOI: 10.1002/pmic.201600126
Abstrakt: Aedes aegypti females ingest sugar or blood to obtain the nutrients needed to maintain cellular homeostasis. During human blood ingestion, female mosquitoes may transmit different viruses such as dengue, yellow fever and, more recently, zika and chikungunya. Here, we report changes in protein expression in the heads of adult female Ae. aegypti mosquitoes in response to the ingestion of blood or sugar. Proteins extracted from the heads of Ae. aegypti fed exclusively on blood (BF) or sugar (SF) were trypsin hydrolyzed (off-gel) and analyzed by the reverse-phase nano-liquid chromatography coupled with hybrid mass spectrometry. A total of 1139 proteins were identified in female heads, representing 7.4% of the predicted proteins in Ae. aegypti genome (total = 15 419 active genes). Gene ontology annotation and categories showed that, in this insect, the head was rich in proteins involved in the metabolic process, proton transport, organelle, macromolecular complex, structural molecule activity, antioxidant activity, and catalytic activity. Our report is the first indicating that many of the annotated genes are translated into functional proteins in heads of adult female Ae. aegypti. Interestingly, we identified 8.7 times more exclusively expressed proteins involved in signal transduction, replication-transcription-translation (5.5 x), and transport (2.9 x) activity in BF than in SF groups. This paper discusses the protein profile of Ae. aegypti female heads and its implications for blood ingestion and carbohydrate intake.
(© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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