Accuracy of a rapid real-time polymerase chain reaction assay for diagnosis of group B Streptococcus colonization in a cohort of HIV-infected pregnant women.

Autor:	Gouvea MIS; a Infectious Diseases Department, Hospital Federal dos Servidores do Estado , Rio de Janeiro , Brazil.; b Instituto Nacional de Infectologia Evandro Chagas - Fiocruz , Rio de Janeiro , Brazil., Joao EC; a Infectious Diseases Department, Hospital Federal dos Servidores do Estado , Rio de Janeiro , Brazil., Teixeira MLB; a Infectious Diseases Department, Hospital Federal dos Servidores do Estado , Rio de Janeiro , Brazil.; b Instituto Nacional de Infectologia Evandro Chagas - Fiocruz , Rio de Janeiro , Brazil., Read JS; c Department of Epidemiology and Biostatistics , University of California at San Francisco , San Francisco , CA , USA., Fracalanzza SEL; d Laboratório de Bacteriologia Médica do Instituto de Microbiologia Prof. Paulo de Góes , Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil., Souza CTV; b Instituto Nacional de Infectologia Evandro Chagas - Fiocruz , Rio de Janeiro , Brazil., Souza MJ; e Department of Bacteriology , Hospital Federal dos Servidores do Estado , Rio de Janeiro , Brazil , and., Torres Filho HM; f Laboratório Richet , Núcleo de Apoio à Pesquisa Clínica , Rio de Janeiro , Brazil., Leite CCF; f Laboratório Richet , Núcleo de Apoio à Pesquisa Clínica , Rio de Janeiro , Brazil., do Brasil PEAA; b Instituto Nacional de Infectologia Evandro Chagas - Fiocruz , Rio de Janeiro , Brazil.
Jazyk:	angličtina
Zdroj:	The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians [J Matern Fetal Neonatal Med] 2017 May; Vol. 30 (9), pp. 1096-1101. Date of Electronic Publication: 2016 Jul 15.
DOI:	10.1080/14767058.2016.1205021
Abstrakt:	Objective: There are limited data regarding Xpert performance to detect Group B Streptococcus (GBS) in HIV-infected pregnant women. We evaluated the accuracy of a rapid real-time polymerase chain reaction (PCR) test in a cohort of HIV-infected women. Methods: At 35-37 weeks of pregnancy, a pair of combined rectovaginal swabs were collected for two GBS assays in a cohort of sequentially included HIV-infected women in Rio de Janeiro: (1) culture; and (2) real-time PCR assay [GeneXpert GBS (Cepheid, Sunnyvale, CA)]. Using culture as the reference, sensitivity, specificity, positive and negative-likelihood ratios were estimated. Results: From June 2012 to February 2015, 337 pregnant women met inclusion criteria. One woman was later excluded, due to failure to obtain a result in the index test; 336 were included in the analyses. The GBS colonization rate was 19.04%. Sensitivity and specificity of the GeneXpert GBS assay were 85.94% (95% CI: 75.38-92.42) and 94.85% (95% CI: 91.55-96.91), respectively. Positive and negative predictive values were 79.71% (95% CI: 68.78-87.51) and 96.63% (95% CI: 93.72-98.22), respectively. Conclusions: GeneXpert GBS is an acceptable test for the identification of GBS colonization in HIV-infected pregnant women and represents a reasonable option to detect GBS colonization in settings where culture is not feasible.
Databáze:	MEDLINE
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