| Autor: |
Serralheiro P; 1 Department of General Surgery, Norfolk and Norwich University Hospital, UK.; 2 Faculdade de Ciências da Saúde, University of Beira Interior, Portugal., Cairrão E; 2 Faculdade de Ciências da Saúde, University of Beira Interior, Portugal., Maia CJ; 2 Faculdade de Ciências da Saúde, University of Beira Interior, Portugal., João M; 2 Faculdade de Ciências da Saúde, University of Beira Interior, Portugal., Almeida CMC; 3 Department of General Surgery, Coimbra University Hospital Centre, Portugal.; 4 Faculdade de Medicina, University of Coimbra, Portugal., Verde I; 2 Faculdade de Ciências da Saúde, University of Beira Interior, Portugal. |
| Abstrakt: |
Objectives Transforming growth factor-beta1 (TGF-β1) may participate in local chronic inflammatory processes in varicose veins and in venous wall structure modifications through regulation of matrix metalloproteinases (MMP) and their inhibitors (tissue inhibitor of metalloproteinase (TIMP)). The aim of this study was to analyze the effect of TGF-β1 in the vein wall, namely on the gene expression of selected MMP, TIMP and TGF-β1 receptors. Methods Healthy vein samples were harvested from eight subjects who underwent coronary bypass graft surgery with great saphenous vein. Each vein sample was divided into two segments, which were cultivated separately in vitro (one of the segments had TGF-β1 added) and then submitted to gene expression analysis. Results In the TGF-β1 supplemented group, there was a general increase in the mean gene expression. Specifically, expression of MMP9, MMP12, TIMP1 and TIMP2 were statistically significant. Conclusion The results of this study demonstrate that the gene expression of MMP9, MMP12, TIMP1 and TIMP2 was influenced by the addition of TGF-β1. These results may be translated to chronic venous insufficiency framework and suggest involvement of TGF-β1 in the vein wall pathology. |
