GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide.

Autor: Lima DC; Laboratório de Biologia Molecular e Genômica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Campus Universitário s/n, Lagoa Nova, Natal, RN, 59072-970 Brazil., Duarte FT; Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte (IFRN), RN, Brazil., Medeiros VK; Colegiado de Medicina, Universidade Federal do Vale do São Francisco, Avenida José de Sá Maniçoba, S/N, Centro, Petrolina, PE, 56304-917, Brazil., Carvalho PC; Laboratório de Proteômica e Engenharia de Proteínas, Instituto Carlos Chagas, Fiocruz, PR, Brazil., Nogueira FC; Unidade Proteômica, Laboratório de Química de Proteínas, Bloco A - sala 543, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos, 149, Cidade Universitária, Rio de Janeiro, RJ, 21941 - 909, Brazil., Araujo GD; Unidade Proteômica, Laboratório de Química de Proteínas, Bloco A - sala 543, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos, 149, Cidade Universitária, Rio de Janeiro, RJ, 21941 - 909, Brazil., Domont GB; Unidade Proteômica, Laboratório de Química de Proteínas, Bloco A - sala 543, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos, 149, Cidade Universitária, Rio de Janeiro, RJ, 21941 - 909, Brazil., Batistuzzo de Medeiros SR; Laboratório de Biologia Molecular e Genômica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Campus Universitário s/n, Lagoa Nova, Natal, RN, 59072-970 Brazil.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2016 Jun 20; Vol. 6, pp. 28174. Date of Electronic Publication: 2016 Jun 20.
DOI: 10.1038/srep28174
Abstrakt: Chromobacterium violaceum is a free-living bacillus with several genes that enables it survival under different harsh environments such as oxidative and temperature stresses. Here we performed a label-free quantitative proteomic study to unravel the molecular mechanisms that enable C. violaceum to survive oxidative stress. To achieve this, total proteins extracted from control and C. violaceum cultures exposed during two hours with 8 mM hydrogen peroxide were analyzed using GeLC-MS proteomics. Analysis revealed that under the stress condition, the bacterium expressed proteins that protected it from the damage caused by reactive oxygen condition and decreasing the abundance of proteins responsible for bacterial growth and catabolism. GeLC-MS proteomics analysis provided an overview of the metabolic pathways involved in the response of C. violaceum to oxidative stress ultimately aggregating knowledge of the response of this organism to environmental stress. This study identified approximately 1500 proteins, generating the largest proteomic coverage of C. violaceum so far. We also detected proteins with unknown function that we hypothesize to be part of new mechanisms related to oxidative stress defense. Finally, we identified the mechanism of clustered regularly interspaced short palindromic repeats (CRISPR), which has not yet been reported for this organism.
Databáze: MEDLINE
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