Anti-bacterial and anti-biofilm activity of probiotic bacteria against oral pathogens.

Autor: Ben Taheur F; Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia., Kouidhi B; College of Applied Medical Sciences, Medical Laboratory Department, Yanbu el Bahr, Taibah University, Al Madinah Al Monawarah, Saudi Arabia., Fdhila K; Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia., Elabed H; Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia., Ben Slama R; Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia., Mahdouani K; Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia., Bakhrouf A; Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia., Chaieb K; College of Sciences, Yanbu el Bahr, Taibah University, Al Madinah Al Monawarah, Saudi Arabia. Electronic address: chaieb_mo@yahoo.fr.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2016 Aug; Vol. 97, pp. 213-20. Date of Electronic Publication: 2016 Jun 15.
DOI: 10.1016/j.micpath.2016.06.018
Abstrakt: In this study, three lactic acid bacteria (LAB), isolated from barley, traditional dried meat and fermented olive were characterized and tested for their anti-bacterial and anti-biofilm activities against oral bacteria. Our results revealed that the tested LAB were γ-hemolytic and were susceptible to four antibiotics. All the strains were resistant to low pH, bile salt, pepsin and pancreatin. Furthermore, FB2 displayed a high aut-oaggregative phenotype (99.54%) while FF2 exhibited the best co-aggregation rate. Concerning the microbial adhesion to solvent, FB2 was the most hydrophobic strain (data obtained with chloroform and n-hexadecane). In addition Pediococcus pentosaceus FB2 and Lactobacillus brevis FF2 displayed a significant inhibitory effect against Streptococcus salivarius B468 (MIC = 10%). Moreover the selected strains were able to inhibit biofilm formation of Bacillus cereus ATCC14579 (MBIC50 = 28.16%) and S. salivarius B468 (MBIC50 = 42.28%). The selected LAB could be considered as candidate probiotics for further application in functional food and mainly in the prevention of oral diseases.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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