Drosophila Condensin II subunit Chromosome-associated protein D3 regulates cell fate determination through non-cell-autonomous signaling.

Autor:	Klebanow LR; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA., Peshel EC; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA., Schuster AT; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA., De K; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA., Sarvepalli K; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA., Lemieux ME; Bioinfo, Plantagenet, ON K0B 1L0, Canada., Lenoir JJ; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA Department of Molecular Biosciences, Northwestern University, Evanston, IL 60201, USA., Moore AW; Disease Mechanism Research Core, RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan., McDonald JA; Division of Biology, Kansas State University, Manhattan, KS 66506, USA., Longworth MS; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA longwom@ccf.org.
Jazyk:	angličtina
Zdroj:	Development (Cambridge, England) [Development] 2016 Aug 01; Vol. 143 (15), pp. 2791-802. Date of Electronic Publication: 2016 Jun 17.
DOI:	10.1242/dev.133686
Abstrakt:	The pattern of the Drosophila melanogaster adult wing is heavily influenced by the expression of proteins that dictate cell fate decisions between intervein and vein during development. dSRF (Blistered) expression in specific regions of the larval wing disc promotes intervein cell fate, whereas EGFR activity promotes vein cell fate. Here, we report that the chromatin-organizing protein CAP-D3 acts to dampen dSRF levels at the anterior/posterior boundary in the larval wing disc, promoting differentiation of cells into the anterior crossvein. CAP-D3 represses KNOT expression in cells immediately adjacent to the anterior/posterior boundary, thus blocking KNOT-mediated repression of EGFR activity and preventing cell death. Maintenance of EGFR activity in these cells depresses dSRF levels in the neighboring anterior crossvein progenitor cells, allowing them to differentiate into vein cells. These findings uncover a novel transcriptional regulatory network influencing Drosophila wing vein development, and are the first to identify a Condensin II subunit as an important regulator of EGFR activity and cell fate determination in vivo. Competing Interests: The authors declare no competing or financial interests. (© 2016. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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