In situ cultured preantral follicles is a useful model to evaluate the effect of anticancer drugs on caprine folliculogenesis.

Autor: Guerreiro DD; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil., Lima LF; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil., Rodrigues GQ; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil., Carvalho Ade A; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil., Castro SV; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil., Campello CC; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil., Pessoa Cdo Ó; Department Pharmacology, Federal University of Ceará, Fortaleza, CE, 60430-270, Brazil., Gadelha CR; Department of Animal Science, Federal University of Ceará, Fortaleza, CE, 60430-270, Brazil., Figueiredo JR; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil., Bordignon V; Department of Animal Science, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada., Rodrigues AP; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil.
Jazyk: angličtina
Zdroj: Microscopy research and technique [Microsc Res Tech] 2016 Aug; Vol. 79 (8), pp. 773-81. Date of Electronic Publication: 2016 Jun 17.
DOI: 10.1002/jemt.22697
Abstrakt: Despite the increase in the incidence of cancer, the number of women who survive cancer treatment is growing. However, one of the principal results of chemotherapy is premature ovarian failure (POF). The aim of this study was to use the in situ culture preantral follicles as an in vitro model to evaluate the toxicity of two anticancer drugs, doxorubicin (DXR) and paclitaxel (PTX), on the integrity and development of ovarian follicles. Fragments of the ovarian cortex of goats were cultured in vitro for 1 or 7 days in α-MEM(+) supplemented with different concentrations of DXR (0.003, 0.03, or 0.3 µg/mL) and PTX (0.001, 0.01, or 0.1 µg/mL). Analyses were performed before and after culture to evaluate tissue integrity by classical histology, apoptosis by TUNEL assay, DNA laddering kit and the detection of activated caspase 3, and DNA damage by the immune detection of phosphorylated histone H2A.x (H2AXph139). Both DXR and PTX reduced the number of morphologically normal primordial and developing follicles. Positive staining for TUNEL and active caspase 3 was detected in all the samples (P < 0.05). Therefore, we propose the in situ culture of caprine preantral follicles as a useful experimental model for assessing the toxic effects of the chemotherapeutic agents on ovarian folliculogenesis. Microsc. Res. Tech. 79:773-781, 2016. © 2016 Wiley Periodicals, Inc.
(© 2016 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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