Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast.

Autor: Hochmal AK; Institute of Plant Biology and Biotechnology, University of Münster, 48143 Münster, Germany., Zinzius K; Institute of Plant Biology and Biotechnology, University of Münster, 48143 Münster, Germany., Charoenwattanasatien R; Institute for Protein Research, Osaka University, Suita Osaka 565-0871, Japan., Gäbelein P; Institute of Plant Biology and Biotechnology, University of Münster, 48143 Münster, Germany., Mutoh R; Institute for Protein Research, Osaka University, Suita Osaka 565-0871, Japan.; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan., Tanaka H; Institute for Protein Research, Osaka University, Suita Osaka 565-0871, Japan.; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan., Schulze S; Institute of Plant Biology and Biotechnology, University of Münster, 48143 Münster, Germany., Liu G; Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, China., Scholz M; Institute of Plant Biology and Biotechnology, University of Münster, 48143 Münster, Germany., Nordhues A; Institute of Plant Biology and Biotechnology, University of Münster, 48143 Münster, Germany., Offenborn JN; Institute of Plant Biology and Biotechnology, University of Münster, 48143 Münster, Germany., Petroutsos D; Centre National Recherche Scientifique, Unité Mixte Recherche 5168, Laboratoire Physiologie Cellulaire et Végétale, F-38054 Grenoble, France.; Commissariat à l'Energie Atomique et Energies Alternatives, l'Institut de Recherches en Technologies et Sciences pour le Vivant, F-38054 Grenoble, France.; Université Grenoble 1, F-38041 Grenoble, France.; Institut National Recherche Agronomique, UMR1200, F-38054 Grenoble, France., Finazzi G; Centre National Recherche Scientifique, Unité Mixte Recherche 5168, Laboratoire Physiologie Cellulaire et Végétale, F-38054 Grenoble, France.; Commissariat à l'Energie Atomique et Energies Alternatives, l'Institut de Recherches en Technologies et Sciences pour le Vivant, F-38054 Grenoble, France.; Université Grenoble 1, F-38041 Grenoble, France.; Institut National Recherche Agronomique, UMR1200, F-38054 Grenoble, France., Fufezan C; Institute of Plant Biology and Biotechnology, University of Münster, 48143 Münster, Germany., Huang K; Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, China., Kurisu G; Institute for Protein Research, Osaka University, Suita Osaka 565-0871, Japan.; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan., Hippler M; Institute of Plant Biology and Biotechnology, University of Münster, 48143 Münster, Germany.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2016 Jun 14; Vol. 7, pp. 11847. Date of Electronic Publication: 2016 Jun 14.
DOI: 10.1038/ncomms11847
Abstrakt: Calcium (Ca(2+)) and redox signalling play important roles in acclimation processes from archaea to eukaryotic organisms. Herein we characterized a unique protein from Chlamydomonas reinhardtii that has the competence to integrate Ca(2+)- and redox-related signalling. This protein, designated as calredoxin (CRX), combines four Ca(2+)-binding EF-hands and a thioredoxin (TRX) domain. A crystal structure of CRX, at 1.6 Å resolution, revealed an unusual calmodulin-fold of the Ca(2+)-binding EF-hands, which is functionally linked via an inter-domain communication path with the enzymatically active TRX domain. CRX is chloroplast-localized and interacted with a chloroplast 2-Cys peroxiredoxin (PRX1). Ca(2+)-binding to CRX is critical for its TRX activity and for efficient binding and reduction of PRX1. Thereby, CRX represents a new class of Ca(2+)-dependent 'sensor-responder' proteins. Genetically engineered Chlamydomonas strains with strongly diminished amounts of CRX revealed altered photosynthetic electron transfer and were affected in oxidative stress response underpinning a function of CRX in stress acclimation.
Databáze: MEDLINE
Externí odkaz: