| Autor: |
Tiyo R; Faculdade Ingá, Maringá, Rodovia PR-317, 6114 CEP 87035-510 Maringá, Paraná, Brazil., de Souza CZ; State University of Maringá, Environmental Parasitology Laboratory, Avenue Colombo, 5790 CEP 87020-9000 Maringá, Paraná, Brazil. carllazangari@gmail.com., Arruda Piovesani AF; State University of Maringá, Environmental Parasitology Laboratory, Avenue Colombo, 5790 CEP 87020-9000 Maringá, Paraná, Brazil., Tiyo BT; State University of Maringá, Environmental Parasitology Laboratory, Avenue Colombo, 5790 CEP 87020-9000 Maringá, Paraná, Brazil., Colli CM; State University of Maringá, Environmental Parasitology Laboratory, Avenue Colombo, 5790 CEP 87020-9000 Maringá, Paraná, Brazil., Marchioro AA; State University of Maringá, Environmental Parasitology Laboratory, Avenue Colombo, 5790 CEP 87020-9000 Maringá, Paraná, Brazil., Gomes ML; State University of Maringá, Environmental Parasitology Laboratory, Avenue Colombo, 5790 CEP 87020-9000 Maringá, Paraná, Brazil., Falavigna-Guilherme AL; State University of Maringá, Environmental Parasitology Laboratory, Avenue Colombo, 5790 CEP 87020-9000 Maringá, Paraná, Brazil. |
| Abstrakt: |
We investigated the presence of Giardia duodenalis cysts and its genotypes in raw leafy vegetables sold in a Brazilian market. These products are different from those sold in most street markets because the producers themselves display and sell their products and rely on specialized technical and sanitary assistance. Vegetable and water samples were collected from 14 (80%) producers who cultivated vegetables that are typically consumed raw for sale at the market, obtained at the market and farms, respectively. A total of 128 samples of leafy greens (chives, parsley, cabbage, arugula, watercress, and chicory) and 14 water samples were analyzed by direct immunofluorescence and PCR techniques. The positive samples were genotyped (GHD gene) using PCR and restriction fragment length polymorphism. The analyses indicated that 16 (12.5%) of 128 samples were positive by PCR, while 1 (0.8%) of 128 samples were positive by immunofluorescence. Giardia cysts were not detected in the water samples obtained at the farms. The molecular technique revealed a genotype with zoonotic potential, which underscores the challenge in the control of giardiasis dissemination via the consumption of raw vegetables. |
