DNA excision repair and double-strand break repair gene polymorphisms and the level of chromosome aberration in children with long-term exposure to radon.

Autor: Larionov AV; a Department of Genetics, Biology Faculty , Kemerovo State University , Kemerovo , Russian Federation ;, Sinitsky MY; a Department of Genetics, Biology Faculty , Kemerovo State University , Kemerovo , Russian Federation ;; b Federal State Budget Scientific Institution, The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences , Kemerovo , Russian Federation ;; c Laboratory of Genome Medicine, Research Institute for Complex Issues of Cardiovascular Diseases , Kemerovo , Russian Federation., Druzhinin VG; a Department of Genetics, Biology Faculty , Kemerovo State University , Kemerovo , Russian Federation ;; b Federal State Budget Scientific Institution, The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences , Kemerovo , Russian Federation ;, Volobaev VP; a Department of Genetics, Biology Faculty , Kemerovo State University , Kemerovo , Russian Federation ;, Minina VI; a Department of Genetics, Biology Faculty , Kemerovo State University , Kemerovo , Russian Federation ;; b Federal State Budget Scientific Institution, The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences , Kemerovo , Russian Federation ;, Asanov MA; b Federal State Budget Scientific Institution, The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences , Kemerovo , Russian Federation ;, Meyer AV; a Department of Genetics, Biology Faculty , Kemerovo State University , Kemerovo , Russian Federation ;, Tolochko TA; a Department of Genetics, Biology Faculty , Kemerovo State University , Kemerovo , Russian Federation ;, Kalyuzhnaya EE; a Department of Genetics, Biology Faculty , Kemerovo State University , Kemerovo , Russian Federation ;
Jazyk: angličtina
Zdroj: International journal of radiation biology [Int J Radiat Biol] 2016 Aug; Vol. 92 (8), pp. 466-74. Date of Electronic Publication: 2016 Jun 10.
DOI: 10.1080/09553002.2016.1186303
Abstrakt: Purpose: To study polymorphic variants of repair genes in people affected by long-term exposure to radon. The chromosome aberration frequency in peripheral blood lymphocytes was used as the biological marker of genotoxicity.
Materials and Methods: Genotyping of 12 single nucleotide polymorphisms in DNA repair genes (APE, XRCC1, OGG1, ADPRT, XpC, XpD, XpG, Lig4 and NBS1) was performed in children with long-term resident exposure to radon. Quantification of the aberrations was performed using light microscopy.
Results: The total frequency of aberrations was increased in carriers of the G/G genotype for the XpD gene (rs13181) polymorphism in recessive model confirmed by the results of ROC-analysis ('satisfactory predictor', AUC = 0.609). Single chromosome fragments frequency was increased in carriers of the G/G genotype in comparison with the T/T genotype. In respect to the total frequency of aberrations, the G/G genotype for the XpG gene (rs17655) polymorphism was also identified as a 'satisfactory predictor' (AUC = 0.605). Carriers of the T/C genotype for the ADPRT gene (rs1136410) polymorphism were characterized by an increased level of single fragments relative to the T/T genotype.
Conclusion: The relationships with several types of cytogenetic damage suggest these three SNP (rs13181, rs17655 and rs1136410) may be considered radiosensitivity markers.
Databáze: MEDLINE
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