Translating microfluidics: Cell separation technologies and their barriers to commercialization.

Autor: Shields CW 4th; NSF Research Triangle Materials Research Science and Engineering Center, Duke University, Durham, North Carolina, 27708.; Department of Biomedical Engineering, Duke University, Durham, North Carolina, 27708., Ohiri KA; NSF Research Triangle Materials Research Science and Engineering Center, Duke University, Durham, North Carolina, 27708.; Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina, 27708., Szott LM; NSF Research Triangle Materials Research Science and Engineering Center, Duke University, Durham, North Carolina, 27708.; Department of Biomedical Engineering, Duke University, Durham, North Carolina, 27708., López GP; NSF Research Triangle Materials Research Science and Engineering Center, Duke University, Durham, North Carolina, 27708.; Department of Biomedical Engineering, Duke University, Durham, North Carolina, 27708.; Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina, 27708.; Center for Biomedical Engineering, Department of Chemical and Biological Engineering, University of New Mexico, Albuquerque, New Mexico, 87131.
Jazyk: angličtina
Zdroj: Cytometry. Part B, Clinical cytometry [Cytometry B Clin Cytom] 2017 Mar; Vol. 92 (2), pp. 115-125. Date of Electronic Publication: 2016 Jul 05.
DOI: 10.1002/cyto.b.21388
Abstrakt: Advances in microfluidic cell sorting have revolutionized the ways in which cell-containing fluids are processed, now providing performances comparable to, or exceeding, traditional systems, but in a vastly miniaturized format. These technologies exploit a wide variety of physical phenomena to manipulate cells and fluid flow, such as magnetic traps, sound waves and flow-altering micropatterns, and they can evaluate single cells by immobilizing them onto surfaces for chemotherapeutic assessment, encapsulate cells into picoliter droplets for toxicity screenings and examine the interactions between pairs of cells in response to new, experimental drugs. However, despite the massive surge of innovation in these high-performance lab-on-a-chip devices, few have undergone successful commercialization, and no device has been translated to a widely distributed clinical commodity to date. Persistent challenges such as an increasingly saturated patent landscape as well as complex user interfaces are among several factors that may contribute to their slowed progress. In this article, we identify several of the leading microfluidic technologies for sorting cells that are poised for clinical translation; we examine the principal barriers preventing their routine clinical use; finally, we provide a prospectus to elucidate the key criteria that must be met to overcome those barriers. Once established, these tools may soon transform how clinical labs study various ailments and diseases by separating cells for downstream sequencing and enabling other forms of advanced cellular or sub-cellular analysis. © 2016 International Clinical Cytometry Society.
(© 2016 International Clinical Cytometry Society.)
Databáze: MEDLINE
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