Acute paraquat exposure determines dose-dependent oxidative injury of multiple organs and metabolic dysfunction in rats: impact on exercise tolerance.

Autor: Novaes RD; Department of Structural Biology, Federal University of Alfenas, Alfenas, MG, Brazil., Gonçalves RV; Department of Animal Biology, Federal University of Viçosa, Viçosa, MG, Brazil., Cupertino MC; Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil., Santos EC; Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil., Bigonha SM; Department of Nutrition and Health, Federal University of Viçosa, Viçosa, MG, Brazil., Fernandes GJ; Department of Anatomy, Federal University of Alfenas, Alfenas, MG, Brazil., Maldonado IR; Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil., Natali AJ; Department of Physical Education, Federal University of Viçosa, Viçosa, MG, Brazil.
Jazyk: angličtina
Zdroj: International journal of experimental pathology [Int J Exp Pathol] 2016 Apr; Vol. 97 (2), pp. 114-24. Date of Electronic Publication: 2016 Jun 09.
DOI: 10.1111/iep.12183
Abstrakt: This study investigated the pathological morphofunctional adaptations related to the imbalance of exercise tolerance triggered by paraquat (PQ) exposure in rats. The rats were randomized into four groups with eight animals each: (a) SAL (control): 0.5 ml of 0.9% NaCl solution; (b) PQ10: PQ 10 mg/kg; (c) PQ20: PQ 20 mg/kg; and (d) PQ30: PQ 30 mg/kg. Each group received a single injection of PQ. After 72 hours, the animals were subjected to an incremental aerobic running test until fatigue in order to determine exercise tolerance, blood glucose and lactate levels. After the next 24 h, lung, liver and skeletal muscle were collected for biometric, biochemical and morphological analyses. The animals exposed to PQ exhibited a significant anticipation of anaerobic metabolism during the incremental aerobic running test, a reduction in exercise tolerance and blood glucose levels as well as increased blood lactate levels during exercise compared to control animals. PQ exposure increased serum transaminase levels and reduced the glycogen contents in liver tissue and skeletal muscles. In the lung, the liver and the skeletal muscle, PQ exposure also increased the contents of malondialdehyde, protein carbonyl, 8-hydroxy-2'-deoxyguanosine, superoxide dismutase and catalase, as well as a structural remodelling compared to the control group. All these changes were dose-dependent. Reduced exercise tolerance after PQ exposure was potentially influenced by pathological remodelling of multiple organs, in which glycogen depletion in the liver and skeletal muscle and the imbalance of glucose metabolism coexist with the induction of lipid, protein and DNA oxidation, a destructive process not counteracted by the upregulation of endogenous antioxidant enzymes.
(© 2016 The Authors. International Journal of Experimental Pathology © 2016 International Journal of Experimental Pathology.)
Databáze: MEDLINE
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