Extensive miRNA expression analysis in craniopharyngiomas.

Autor: Samis J; Division of Endocrinology, Ann and Robert H. Lurie Children's Hospital of Chicago, 225 E Chicago Ave., Chicago, IL, 60611, USA.; Feinberg School of Medicine, Northwestern University, 303 E Chicago Ave, Chicago, IL, 60611, USA., Vanin EF; Cancer Biology and Epigenomics, Stanley Manne Children's Research Institute, 2430 North Halsted St., Chicago, IL, 60614, USA., Sredni ST; Feinberg School of Medicine, Northwestern University, 303 E Chicago Ave, Chicago, IL, 60611, USA. ssredni@luriechildrens.org.; Cancer Biology and Epigenomics, Stanley Manne Children's Research Institute, 2430 North Halsted St., Chicago, IL, 60614, USA. ssredni@luriechildrens.org.; Division of Pediatric Neurosurgery, Ann and Robert H. Lurie Children's Hospital of Chicago, 225 E Chicago Ave., Chicago, IL, 60611, USA. ssredni@luriechildrens.org., de Bonaldo Mde F; Cancer Biology and Epigenomics, Stanley Manne Children's Research Institute, 2430 North Halsted St., Chicago, IL, 60614, USA., Costa FF; Cancer Biology and Epigenomics, Stanley Manne Children's Research Institute, 2430 North Halsted St., Chicago, IL, 60614, USA., Tomita T; Feinberg School of Medicine, Northwestern University, 303 E Chicago Ave, Chicago, IL, 60611, USA.; Division of Pediatric Neurosurgery, Ann and Robert H. Lurie Children's Hospital of Chicago, 225 E Chicago Ave., Chicago, IL, 60611, USA., Habiby R; Division of Endocrinology, Ann and Robert H. Lurie Children's Hospital of Chicago, 225 E Chicago Ave., Chicago, IL, 60611, USA.; Feinberg School of Medicine, Northwestern University, 303 E Chicago Ave, Chicago, IL, 60611, USA., Zimmerman D; Division of Endocrinology, Ann and Robert H. Lurie Children's Hospital of Chicago, 225 E Chicago Ave., Chicago, IL, 60611, USA.; Feinberg School of Medicine, Northwestern University, 303 E Chicago Ave, Chicago, IL, 60611, USA., Soares MB; Cancer Biology and Epigenomics, Stanley Manne Children's Research Institute, 2430 North Halsted St., Chicago, IL, 60614, USA.; Division of Pediatric Neurosurgery, Ann and Robert H. Lurie Children's Hospital of Chicago, 225 E Chicago Ave., Chicago, IL, 60611, USA.; University of Illinois College of Medicine at Peoria, One Illini Drive, Peoria, IL, 61605, USA.
Jazyk: angličtina
Zdroj: Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery [Childs Nerv Syst] 2016 Sep; Vol. 32 (9), pp. 1617-24. Date of Electronic Publication: 2016 Jun 07.
DOI: 10.1007/s00381-016-3131-1
Abstrakt: Purpose: Craniopharyngiomas are benign tumors of the sellar or parasellar regions. They arise from the remnants of Rathke's pouch and are considered a "developmental disease." microRNAs are short non-coding RNAs that play a key regulatory role in the control of expression of entire gene networks. We performed an extensive analysis of miRNAs in craniopharyngiomas aiming to identify a miRNA expression signature that might aid in the prognosis of disease progression and outcome.
Methods: Thirty-seven craniopharyngioma samples from twenty-three patients, ten age-matched controls from autopsy, and ten infant controls from the developing pituitary from autopsy were evaluated for the expression of 754 miRNAs using TaqMan® Low Density Arrays (TLDAs) v2.0 (Applied Biosystems, Foster City, CA).
Results: Among the most differentially expressed miRNAs, downregulation of miR-132 appears to be a marker of aggressiveness and also plays a role in epithelial-mesenchymal transition.
Conclusions: This is the first time that an extensive study of miRNA expression has been performed in craniopharyngiomas. Further research needs to be performed to investigate the potential role of miR-132 in the development and progression of craniopharyngiomas, and its value as a prognostic marker of aggressiveness.
Databáze: MEDLINE
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